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Literature summary for 4.2.3.75 extracted from

  • Luecker, J.; Bowen, P.; Bohlmann, J.
    Vitis vinifera terpenoid cyclases: functional identification of two sesquiterpene synthase cDNAs encoding (+)-valencene synthase and (-)-germacrene D synthase and expression of mono- and sesquiterpene synthases in grapevine flowers and berries (2004), Phytochemistry, 65, 2649-2659.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis of Vitis vinifera cv. Gewürztraminer, phylogenetic tree, expression in Escherichia coli strain BL21(DE3)-RIL Vitis vinifera
expression in Escherichia coli Vitis vinifera

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Vitis vinifera

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64300
-
x * 64300, calculated Vitis vinifera

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-trans,6-trans-farnesyl diphosphate Vitis vinifera
-
(-)-(7S)-germacrene D + diphosphate
-
?
additional information Vitis vinifera cell terpenoid profile determination ?
-
?

Organism

Organism UniProt Comment Textmining
Vitis vinifera Q6Q3H3
-
-
Vitis vinifera Q6Q3H3 cv. Chardonnay and cv. Gewürztraminer
-

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
Vitis vinifera
-
flower strongest hybridization signals with RNA from flower budsand strongly reduced transcript levels in pre- and postanthesis open flowers and in set flowers at early fruit onset Vitis vinifera
-
flower bud
-
Vitis vinifera
-
fruit young Vitis vinifera
-
leaf vegetative tissues of young leaves Vitis vinifera
-
additional information no transcripts in fruit Vitis vinifera
-
stem green stem Vitis vinifera
-
tendril
-
Vitis vinifera
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate
-
Vitis vinifera (-)-germacrene D + diphosphate plus about 8% dleta-cadinene ?
2-trans,6-trans-farnesyl diphosphate
-
Vitis vinifera (-)-(7S)-germacrene D + diphosphate
-
?
2-trans,6-trans-farnesyl diphosphate strictly enantio-specific reaction of (-)-germacrene D synthase Vitis vinifera (-)-(7S)-germacrene D + diphosphate GC-MS product analysis ?
additional information cell terpenoid profile determination Vitis vinifera ?
-
?
additional information no substrate: geranyl diphosphate or geranylgeranyl diphosphate Vitis vinifera ?
-
?

Subunits

Subunits Comment Organism
? x * 64300, calculated Vitis vinifera

Synonyms

Synonyms Comment Organism
(-)-germacrene D synthase
-
Vitis vinifera
GerD
-
Vitis vinifera
terpenoid cyclase
-
Vitis vinifera
TPS
-
Vitis vinifera
VitiM1610
-
Vitis vinifera

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Vitis vinifera

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Vitis vinifera

pI Value

Organism Comment pI Value Maximum pI Value
Vitis vinifera calculated
-
4.5