Literature summary for 4.2.3.75 extracted from

Prosser, I.; Altug, I.G.; Phillips, A.L.; Koenig, W.A.; Bouwmeester, H.J.; Beale, M.H.
Enantiospecific (+)- and (-)-germacrene D synthases, cloned from goldenrod, reveal a functionally active variant of the universal isoprenoid-biosynthesis aspartate-rich motif (2004), Arch. Biochem. Biophys., 432, 136-144.

Search for more literature for 4.2.3.75

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Solidago canadensis

Protein Variants

Protein Variants	Comment	Organism
D303E	less than 0.25% of wild-type activity	Solidago canadensis
D303N	mimicking of corresponding amino acid in (+)-germacrene D synthase Sc11. 10% of the wild-type, activity, reduction in catalytic constant by a factor of 10, with no change in Km of farnesyl diphosphate, product composition or chirality	Solidago canadensis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0029	-	(2E,6E)-farnesyl diphosphate	25°C, pH not specified in the publication	Solidago canadensis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required. Bi-dentate Mg2+-binding by residue 303 is not obligatory	Solidago canadensis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
65163	-	x * 65163, calculated	Solidago canadensis

Organism

Organism	UniProt	Comment	Textmining
Solidago canadensis	Q70EZ6	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(2E,6E)-farnesyl diphosphate	-	Solidago canadensis	(-)-germacrene D + diphosphate	-	?

Subunits

Subunits	Comment	Organism
?	x * 65163, calculated	Solidago canadensis

Synonyms

Synonyms	Comment	Organism
Sc19	-	Solidago canadensis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.03	-	(2E,6E)-farnesyl diphosphate	25°C, pH not specified in the publication	Solidago canadensis

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
10.3	-	(2E,6E)-farnesyl diphosphate	25°C, pH not specified in the publication	Solidago canadensis

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Release 2023.1 (January 2023)