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Literature summary for 4.2.3.71 extracted from

  • Little, D.B.; Croteau, R.B.
    Alteration of product formation by directed mutagenesis and truncation of the multiple-product sesquiterpene synthases delta-selinene synthase and gamma-humulene synthase (2002), Arch. Biochem. Biophys., 402, 120-135.
    View publication on PubMed
Search for more literature for 4.2.3.71

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Abies grandis

Protein Variants

Protein Variants Comment Organism
D331A mutation results in main products 24.6% delta-amorphene, 12.9 beta-bisabolene, 22.0% germacrene A Abies grandis
D331E mutation results in main products 16.6% beta-bisabolene, 29.3% germacrene A, 9.3% alpha-bisabolene Abies grandis
D475A mutation results in main products 16.6% beta-bisabolene, 29.1% germacrene A, 8.8% alpha-bisabolene Abies grandis
D475E mutation results in main products 13.4% siberene, 27.4% amorphene, 9.1% beta-bisabolene, 10.9% germacrene A Abies grandis
D475N mutation results in main products 16.8% siberene, 34.7% amorphene, 8.2% beta-bisabolene, 6.8% germacrene A Abies grandis
D566N mutation results in main products 20.6% beta-bisabolene, 29.1% germacrene A, 8.4% alpha-bisabolene Abies grandis
H16A mutation results in main products 2.0% delta-selinene, 19.5% germacrene A, 33.7% germacrene C, 21.9% germacrene B Abies grandis
H17A mutation results in main products 1.5% delta-selinene, 19.8% germacrene A, 34.0% germacrene C, 21.8% germacrene B Abies grandis
additional information truncation at residue R11 results in main products 8.7% delta-selinene, 17.7% germacrene A, 21.8% germacrene C, 32.4% germacrene B. Truncation at residue H16 results in main products 1.8% delta-selinene, 17.0% germacrene A, 27.6% germacrene C, 19.7% germacrene B, 18.0% gamma-humulene. Truncation at residue W21 results in main products 23.4% beta-bisabolene, 24.4% germacrene A, 15.2% germacrene C, 4.7% germacrene B, 26.4% gamma-humulene Abies grandis
R11A mutation results in main products 2.5% delta-selinene, 18.2% germacrene A, 33.2% germacrene C, 26.5% germacrene B Abies grandis
R12A mutation results in main products 3.4% delta-selinene, 17.7% germacrene A, 35.8% germacrene C, 28.1% germacrene B Abies grandis
Y552F mutation results in main products 9.1% dleta-selinene, 17.0% germacrene A, 22.9% germacrene C, 33.4% germacrene B Abies grandis

Organism

Organism UniProt Comment Textmining
Abies grandis O64404
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Purification (Commentary)

Purification (Comment) Organism
partial Abies grandis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate
-
 Abies grandis germacrene B + diphosphate main products are 12.3% delta-selinene, 17.2% germacrene A, 14.2% germacrene C, 33.2% germacrene B ?