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Literature summary for 4.2.3.70 extracted from
- Purification and characterization of the sesquiterpene cyclase patchoulol synthase from Pogostemon cablin (1990), Arch. Biochem. Biophys., 282, 58-64.
General Stability
| General Stability | Organism |
|---|---|
| unstable in crude extracts, losing 80% activity in 6 h at room temperature and 40% activity in 24 h at 0-4°C. Addition of dithiothreitol is essential, and Na2HPO4 markedly stabilizes | Pogostemon cablin |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0068 | - |
(2E,6E)-farnesyl diphosphate | pH 6.5, temperature not specified in the publication | Pogostemon cablin |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required, KM-value 1.7 mM, other divalent metal ions are ineffective | Pogostemon cablin | |
| additional information | other divalent metal ions than Mg2+ are ineffective | Pogostemon cablin |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
2 * 40000, SDS-PAGE | Pogostemon cablin |
| 80000 | - |
gel filtration | Pogostemon cablin |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pogostemon cablin | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pogostemon cablin |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Pogostemon cablin | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 180 | - |
pH 6.5, temperature not specified in the publication | Pogostemon cablin |
Storage Stability
| Storage Stability | Organism |
|---|---|
| maximum stability in the presence of Mg2+ above 10 mM and 10% (v/v) glycerol | Pogostemon cablin |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2E,6E)-farnesyl diphosphate + H2O | - |
Pogostemon cablin | patchoulol + diphosphate | further products are cyclic olefins alpha- and beta-patchoulene, alpha-bulnesene, and alpha-guiaene | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 40000, SDS-PAGE | Pogostemon cablin |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.7 | - |
- |
Pogostemon cablin |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.7 | - |
half-maximal activity | Pogostemon cablin |
| 7.7 | - |
half-maximal activity | Pogostemon cablin |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Pogostemon cablin | isoelectric focusing | - |
5 |
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