Literature summary for 4.2.3.69 extracted from

Tholl, D.; Chen, F.; Petri, J.; Gershenzon, J.; Pichersky, E.
Two sesquiterpene synthases are responsible for the complex mixture of sesquiterpenes emitted from Arabidopsis flowers (2005), Plant J., 42, 757-771.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Arabidopsis thaliana

Protein Variants

Protein Variants	Comment	Organism
additional information	full-length cDNA of At5g44630 expressed in Escherichia coli results in a protein that accepts farnesyl diphosphate as a substrate and converts it into over 15 different sesquiterpenes. The enzymatically formed compounds are identical to the floral group B sesquiterpenes and present in similar ratios as those found in the headspace of Arabidopsis thaliana flowers	Arabidopsis thaliana

Inhibitors

Inhibitors	Comment	Organism	Structure
EDTA	1 mM, complete loss of activity	Arabidopsis thaliana

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0012	-	(2E,6E)-farnesyl diphosphate	pH not specified in the publication, temperature not specified in the publication	Arabidopsis thaliana

Metals/Ions

Metals/Ions	Comment	Organism	Structure
additional information	divalent cation required	Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
64908	-	x * 64908, calculated	Arabidopsis thaliana

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	Q4KSH9	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
flower	intrafloral nectaries, exclusive expression in flower	Arabidopsis thaliana	-
nectary	-	Arabidopsis thaliana	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(2E,6E)-farnesyl diphosphate	-	Arabidopsis thaliana	(+)-alpha-barbatene + diphosphate	further products are: (+)-thujopsene + isobazzanene + (+)-beta-barbatene + E -beta-farnesene + beta-acoradiene + (+)-beta-chamigrene + alpha-zingiberene + alpha-cuprenene + alpha-chamigrene + ()-cuparene + ()-?-bisabolene + beta-sesquiphellandrene + delta-cuprenene	?
additional information	in vitro catalyses the reaction of geranyl diphosphate to myrcene + limonene + (Z)-beta-ocimene + (E)-beta-ocimene + terpinolene + alpha-terpineol. Formation of these compounds by the enzyme in vivo is rather unlikely, as the protein lacks a transit peptide and is therefore not expected to be present in plastids, where GPP is thought to be produced	Arabidopsis thaliana	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 64908, calculated	Arabidopsis thaliana

Synonyms

Synonyms	Comment	Organism
At5g44630	-	Arabidopsis thaliana

pI Value

Organism	Comment	pI Value Maximum	pI Value
Arabidopsis thaliana	calculated	-	5.2

General Information

General Information	Comment	Organism
physiological function	the volatile blend from flowers of an T-DNA insertion line contains only group A sesquiterpenes, including (E)-beta-caryophyllene, in similar ratios as the wild type, but none of the group B sesquiterpene compounds	Arabidopsis thaliana

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Release 2023.1 (January 2023)