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Literature summary for 4.2.3.64 extracted from

  • Nakano, C.; Tezuka, T.; Horinouchi, S.; Ohnishi, Y.
    Identification of the SGR6065 gene product as a sesquiterpene cyclase involved in (+)-epicubenol biosynthesis in Streptomyces griseus (2012), J. Antibiot., 65, 551-558.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Streptomyces griseus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000254
-
(2E,6E)-farnesyl diphosphate pH not specified in the publication, temperature not specified in the publication Streptomyces griseus

Organism

Organism UniProt Comment Textmining
Streptomyces griseus B1W477
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate + H2O
-
Streptomyces griseus (+)-epicubenol + diphosphate
-
?

Synonyms

Synonyms Comment Organism
gecA
-
Streptomyces griseus
SGR_6065
-
Streptomyces griseus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.026
-
(2E,6E)-farnesyl diphosphate pH not specified in the publication, temperature not specified in the publication Streptomyces griseus

Expression

Organism Comment Expression
Streptomyces griseus (+)-epicubenol production is not controlled by the microbial hormone A-factor that induces morphological differentiation and production of several secondary metabolites in Streptomyces griseus additional information

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
100
-
(2E,6E)-farnesyl diphosphate pH not specified in the publication, temperature not specified in the publication Streptomyces griseus