Literature summary for 4.2.3.61 extracted from

Starks, C.; Back, K.; Chappell, J.; Noel, J.
Structural basis for cyclic terpene biosynthesis by tobacco 5-epi-aristolochene synthase (1997), Science, 277, 1815-1820.

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Nicotiana tabacum

Crystallization (Commentary)

Crystallization (Comment)	Organism
to 2.2-2.8 A resolution, of TEAS alone and in complexes with two different substrate analogs. TEAS consists entirely of alpha-helices and short connecting loops and turns, and is organized into two structural domains. Two Mg2+ ions are coordinated on opposite sides of the entrance to the active site pocket, and constitute a diphosphate binding site. Asp301 coordinates a Mg2+ in the native TEAS structure, and the side chain carboxyl of Glu379 provides a longer range interaction. Asp305 provides an additional coordination bond in the enzyme with substrate analogs bound. Asp301 and Asp305 are part of a -DDXXD- sequence. Asp301 directly contacts Mg2+, whereas Asp302 demonstrates no direct metal coordination. The side chains of Asp444, Thr448, Glu452, and one water molecule the second coordinate Mg2+. In the native TEAS structure, the A-C and J-K loops and the residues NH2-terminal of residue 36 are disordered	Nicotiana tabacum

Crystallization (Comment)

Organism

to 2.2-2.8 A resolution, of TEAS alone and in complexes with two different substrate analogs. TEAS consists entirely of alpha-helices and short connecting loops and turns, and is organized into two structural domains. Two Mg2+ ions are coordinated on opposite sides of the entrance to the active site pocket, and constitute a diphosphate binding site. Asp301 coordinates a Mg2+ in the native TEAS structure, and the side chain carboxyl of Glu379 provides a longer range interaction. Asp305 provides an additional coordination bond in the enzyme with substrate analogs bound. Asp301 and Asp305 are part of a -DDXXD- sequence. Asp301 directly contacts Mg2+, whereas Asp302 demonstrates no direct metal coordination. The side chains of Asp444, Thr448, Glu452, and one water molecule the second coordinate Mg2+. In the native TEAS structure, the A-C and J-K loops and the residues NH2-terminal of residue 36 are disordered

Nicotiana tabacum

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	presence of 2 Mg2+ ions	Nicotiana tabacum

Organism

Organism	UniProt	Comment	Textmining
Nicotiana tabacum	Q40577	-	-

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Release 2023.1 (January 2023)