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Literature summary for 4.2.3.5 extracted from

  • Ely, F.; Nunes, J.E.; Schroeder, E.K.; Frazzon, J.; Palma, M.S.; Santos, D.S.; Basso, L.A.
    The Mycobacterium tuberculosis Rv2540c DNA sequence encodes a bifunctional chorismate synthase (2008), BMC Biochem., 9, 13.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.3.5

Application

Application Comment Organism
drug development enzymes of shikimate pathway are attractive targets to the development of antitubercular agents Mycobacterium tuberculosis

Cloned(Commentary)

Cloned (Comment) Organism
cloning and expression in Escherichia coli strain Rosetta(DE3) in the absence of IPTG induction, heterologous overexpression of the MtCS protein Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41800
-
 determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis and electrospray mass spectrometry Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5-O-(1-carboxyvinyl)-3-phosphoshikimate Mycobacterium tuberculosis seventh enzyme of the shikimate pathway, catalyzes the NADH- and FMN-dependent synthesis of chorismate, a precursor of aromatic amino acids, naphthoquinones, menaquinones, and mycobactins chorismate + phosphate
-
 r

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by using a 3-step purification (anion exchange, hydrophobic interaction and anion exchange chromatography) Mycobacterium tuberculosis

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Mycobacterium tuberculosis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 bifunctionality is determined by measurements of both chorismate synthase and NADH:FMN oxidoreductase activities Mycobacterium tuberculosis
additional information
-
 in the presence of oxygen the CS activity is limited by the reoxidation of FMNred cofactor Mycobacterium tuberculosis
0.004
-
 CS activity Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-O-(1-carboxyvinyl)-3-phosphoshikimate seventh enzyme of the shikimate pathway, catalyzes the NADH- and FMN-dependent synthesis of chorismate, a precursor of aromatic amino acids, naphthoquinones, menaquinones, and mycobactins Mycobacterium tuberculosis chorismate + phosphate
-
 r

Subunits

Subunits Comment Organism
dimer in solution, determined by gel filtration chromatography Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
5-O-(1-carboxyvinyl)-3-phosphoshikimate phosphate lyase
-
 Mycobacterium tuberculosis
aroF
-
 Mycobacterium tuberculosis
chorismate synthase
-
 Mycobacterium tuberculosis
CS
-
 Mycobacterium tuberculosis
MtCS
-
 Mycobacterium tuberculosis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at, CS activity and FMN-reductase activity Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0083
-
 NADH kinetics of reduction of the MtCS-bound FMNox is determined using fixed amount of holoenzyme and varying levels of NADH Mycobacterium tuberculosis

Cofactor

Cofactor Comment Organism Structure
FMN reduced form Mycobacterium tuberculosis