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Literature summary for 4.2.3.5 extracted from

  • Kitzing, K.; Auweter, S.; Amrhein, N.; Macheroux, P.
    Mechanism of chorismate synthase. Role of the two invariant histidine residues in the active site (2004), J. Biol. Chem., 279, 9451-9461.
    View publication on PubMed
Search for more literature for 4.2.3.5

Protein Variants

Protein Variants Comment Organism
H106A mutant enzyme with 20fold reduced activity Neurospora crassa
H17A mutant enzyme with 10fold reduced activity Neurospora crassa

Inhibitors

Inhibitors Comment Organism Structure
diethyl dicarbonate
-
 Neurospora crassa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.043
-
 NADPH FMN:NADPH oxidoreductase activity, wild-type enzyme Neurospora crassa
0.11
-
 NADPH FMN:NADPH oxidoreductase activity, mutant enzyme H17A Neurospora crassa
0.28
-
 NADPH FMN:NADPH oxidoreductase activity, mutant enzyme H106A Neurospora crassa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
O5-(1-carboxyvinyl)-3-phosphoshikimate Neurospora crassa last step in shikimate pathway chorismate + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Neurospora crassa
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
FMN + NADPH
-
 Neurospora crassa ?
-
 ?
additional information the enzyme also shows FMN:NADPH oxidoreductase activity Neurospora crassa ?
-
 ?
O5-(1-carboxyvinyl)-3-phosphoshikimate
-
 Neurospora crassa chorismate + phosphate
-
 ?
O5-(1-carboxyvinyl)-3-phosphoshikimate last step in shikimate pathway Neurospora crassa chorismate + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
CS
-
 Neurospora crassa

Cofactor

Cofactor Comment Organism Structure
FMNH2 absolute requirement for FMNH2, which is not consumed during the reaction Neurospora crassa