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Literature summary for 4.2.3.5 extracted from
- Chorismate synthase from the hyperthermophile Thermotoga maritima combines thermostability and increased rigidity with catalytic and spectral properties similar to mesophilic counterparts (2001), J. Biol. Chem., 276, 18052-18059.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Thermotoga maritima |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 41754 | - |
4 * 41754, calculation from amino acid sequence | Thermotoga maritima |
| 42000 | - |
4 * 42000, SDS-PAGE | Thermotoga maritima |
| 200000 | - |
non-denaturing PAGE in presence of O5-(1-carboxyvinyl)-3-phosphoshikimate and FMN | Thermotoga maritima |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| O5-(1-carboxyvinyl)-3-phosphoshikimate | Thermotoga maritima | enzyme of the shikimate pathway | chorismate + phosphate | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermotoga maritima | Q9WYI2 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Thermotoga maritima |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | monofunctional enzyme that does not have an intrinsic ability to reduce the FMN cofactor | Thermotoga maritima | ? | - |
? | |
| O5-(1-carboxyvinyl)-3-phosphoshikimate | - |
Thermotoga maritima | chorismate + phosphate | - |
? | |
| O5-(1-carboxyvinyl)-3-phosphoshikimate | enzyme of the shikimate pathway | Thermotoga maritima | chorismate + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 42000, SDS-PAGE | Thermotoga maritima |
| tetramer | 4 * 41754, calculation from amino acid sequence | Thermotoga maritima |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 92 | - |
melting temperature above | Thermotoga maritima |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FMN | cofactor | Thermotoga maritima | |
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Release 2023.1 (January 2023)
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