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Literature summary for 4.2.3.48 extracted from

  • Green, S.A.; Chen, X.; Nieuwenhuizen, N.J.; Matich, A.J.; Wang, M.Y.; Bunn, B.J.; Yauk, Y.K.; Atkinson, R.G.
    Identification, functional characterization, and regulation of the enzyme responsible for floral (E)-nerolidol biosynthesis in kiwifruit (Actinidia chinensis) (2012), J. Exp. Bot., 63, 1951-1967.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.3.48

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0008
-
 (2E,6E)-farnesyl diphosphate pH 7.5, 22°C, presence of Mg2+ Actinidia chinensis
0.0019
-
 geranyl diphosphate pH 7.5, 22°C, presence of Mg2+ Actinidia chinensis

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Actinidia chinensis 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ Km value 0.117 mM Actinidia chinensis
Mn2+ Km value 0.014 mM Actinidia chinensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65400
-
 x * 65400, calculated Actinidia chinensis

Organism

Organism UniProt Comment Textmining
Actinidia chinensis H9M5U5
-
-

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
 Actinidia chinensis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate + H2O
-
 Actinidia chinensis (3S,6E)-nerolidol + diphosphate predominant enantiomer produced ?
geranyl diphosphate + H2O 58% of the velocity with (2E,6E)-farnesyl diphosphate Actinidia chinensis (S)-linalool + diphosphate
-
 ?
geranylgeranyl diphosphate + H2O 10% of the velocity with (2E,6E)-farnesyl diphosphate Actinidia chinensis (E,E)-geranyllinalool + diphosphate
-
 ?
additional information no substrate: dimethylallyl diphosphate Actinidia chinensis ?
-
 ?

Subunits

Subunits Comment Organism
? x * 65400, calculated Actinidia chinensis

Synonyms

Synonyms Comment Organism
NES1
-
 Actinidia chinensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.03
-
 geranyl diphosphate pH 7.5, 22°C, presence of Mn2+ Actinidia chinensis
0.13
-
 geranyl diphosphate pH 7.5, 22°C, presence of Mg2+ Actinidia chinensis
0.24
-
 (2E,6E)-farnesyl diphosphate pH 7.5, 22°C, presence of Mg2+ Actinidia chinensis

Expression

Organism Comment Expression
Actinidia chinensis peak expression of enzyme correlates with peak (E)-nerolidol, but not linalool accumulation in flowers additional information

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
69
-
 geranyl diphosphate pH 7.5, 22°C, presence of Mg2+ Actinidia chinensis
300
-
 (2E,6E)-farnesyl diphosphate pH 7.5, 22°C, presence of Mg2+ Actinidia chinensis