KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0026 | - |
(2E,6E)-farnesyl diphosphate | accession Columbia, 30°C, pH not specified in the publication | Arabidopsis thaliana | |
0.004 | - |
(2E,6E)-farnesyl diphosphate | accession Wassilewskija, 30°C, pH not specified in the publication | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | plastidial transit peptide of 25 amino acids, and fluoresence studies using GPF-fusion protein, accession Wassilewskija | Arabidopsis thaliana | 9507 | - |
cytosol | - |
Arabidopsis thaliana | 5829 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | A4FVP2 | isoform TPS03 | - |
Arabidopsis thaliana | P0CJ43 | isoform TPS02 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | expression occurs constitutively in floral tissues | Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate | - |
Arabidopsis thaliana | (3E,6E)-alpha-farnesene + diphosphate | - |
? | |
additional information | enzyme shows both (E)-beta-ocimene and (E,E)-alpha-farnesene synthase activities | Arabidopsis thaliana | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TPS02 | - |
Arabidopsis thaliana |
TPS03 | - |
Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0045 | - |
(2E,6E)-farnesyl diphosphate | accession Wassilewskija, 30°C, pH not specified in the publication | Arabidopsis thaliana | |
0.0048 | - |
(2E,6E)-farnesyl diphosphate | accession Columbia, 30°C, pH not specified in the publication | Arabidopsis thaliana |
Organism | Comment | Expression |
---|---|---|
Arabidopsis thaliana | expression is induced in leaves by elicitor and insect treatment | up |
General Information | Comment | Organism |
---|---|---|
physiological function | differences in the relative emissions of (E)-beta-ocimene and (E,E)-alpha-farnesene from accession Wassilewskija, a high-(E)-beta-ocimene emitter, and accession Columbia, a trace-(E)-beta-ocimene emitter, are attributed to allelic variation of closely related, tandem-duplicated terpene synthase genes, TPS02 and TPS03. The Wassilewskija genome contains a functional allele of TPS02 but not of TPS03, while the opposite is the case for Columbia. Recombinant proteins of the functional Wassilewskija TPS02 and Columbia TPS03 genes both show (E)-beta-ocimene and (E,E)-alpha-farnesene synthase activities. Differential subcellular compartmentalization of the two enzymes in plastids and the cytosol is responsible for the ecotype-specific differences in (E)-beta-ocimene/(E,E)-alpha-farnesene emission | Arabidopsis thaliana |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.12 | - |
(2E,6E)-farnesyl diphosphate | accession Wassilewskija, 30°C, pH not specified in the publication | Arabidopsis thaliana | |
1.82 | - |
(2E,6E)-farnesyl diphosphate | accession Columbia, 30°C, pH not specified in the publication | Arabidopsis thaliana |