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Literature summary for 4.2.3.46 extracted from

  • Huang, M.; Abel, C.; Sohrabi, R.; Petri, J.; Haupt, I.; Cosimano, J.; Gershenzon, J.; Tholl, D.
    Variation of herbivore-induced volatile terpenes among Arabidopsis ecotypes depends on allelic differences and subcellular targeting of two terpene synthases, TPS02 and TPS03 (2010), Plant Physiol., 153, 1293-1310.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0026
-
(2E,6E)-farnesyl diphosphate accession Columbia, 30°C, pH not specified in the publication Arabidopsis thaliana
0.004
-
(2E,6E)-farnesyl diphosphate accession Wassilewskija, 30°C, pH not specified in the publication Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast plastidial transit peptide of 25 amino acids, and fluoresence studies using GPF-fusion protein, accession Wassilewskija Arabidopsis thaliana 9507
-
cytosol
-
Arabidopsis thaliana 5829
-

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana A4FVP2 isoform TPS03
-
Arabidopsis thaliana P0CJ43 isoform TPS02
-

Source Tissue

Source Tissue Comment Organism Textmining
flower expression occurs constitutively in floral tissues Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate
-
Arabidopsis thaliana (3E,6E)-alpha-farnesene + diphosphate
-
?
additional information enzyme shows both (E)-beta-ocimene and (E,E)-alpha-farnesene synthase activities Arabidopsis thaliana ?
-
?

Synonyms

Synonyms Comment Organism
TPS02
-
Arabidopsis thaliana
TPS03
-
Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0045
-
(2E,6E)-farnesyl diphosphate accession Wassilewskija, 30°C, pH not specified in the publication Arabidopsis thaliana
0.0048
-
(2E,6E)-farnesyl diphosphate accession Columbia, 30°C, pH not specified in the publication Arabidopsis thaliana

Expression

Organism Comment Expression
Arabidopsis thaliana expression is induced in leaves by elicitor and insect treatment up

General Information

General Information Comment Organism
physiological function differences in the relative emissions of (E)-beta-ocimene and (E,E)-alpha-farnesene from accession Wassilewskija, a high-(E)-beta-ocimene emitter, and accession Columbia, a trace-(E)-beta-ocimene emitter, are attributed to allelic variation of closely related, tandem-duplicated terpene synthase genes, TPS02 and TPS03. The Wassilewskija genome contains a functional allele of TPS02 but not of TPS03, while the opposite is the case for Columbia. Recombinant proteins of the functional Wassilewskija TPS02 and Columbia TPS03 genes both show (E)-beta-ocimene and (E,E)-alpha-farnesene synthase activities. Differential subcellular compartmentalization of the two enzymes in plastids and the cytosol is responsible for the ecotype-specific differences in (E)-beta-ocimene/(E,E)-alpha-farnesene emission Arabidopsis thaliana

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.12
-
(2E,6E)-farnesyl diphosphate accession Wassilewskija, 30°C, pH not specified in the publication Arabidopsis thaliana
1.82
-
(2E,6E)-farnesyl diphosphate accession Columbia, 30°C, pH not specified in the publication Arabidopsis thaliana