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Literature summary for 4.2.3.46 extracted from

  • Nieuwenhuizen, N.J.; Wang, M.Y.; Matich, A.J.; Green, S.A.; Chen, X.; Yauk, Y.-K.; Beuning, L.L.; Atkinson, R.G.
    Two terpene synthases are responsible for the major sesquiterpenes emitted from the flowers of kiwifruit (Actinidia deliciosa) (2009), J. Exp. Bot., 60, 3203-3219.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.3.46

Cloned(Commentary)

Cloned (Comment) Organism
heterologously expressed in Escherichia coli Actinidia deliciosa

Inhibitors

Inhibitors Comment Organism Structure
Mn2+ above 0.03 mM Actinidia deliciosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0028
-
 geranyl diphosphate pH 7.5 Actinidia deliciosa
0.0095
-
 (2E,6E)-farnesyl diphosphate pH 7.5 Actinidia deliciosa

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Actinidia deliciosa 5829
-
additional information AdAFS1 protein sequences lacks predicted N-terminal transit peptide-like sequences for chloroplast targeting Actinidia deliciosa
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ Km: 0.248 mM, divalent cation required, preference for Mg2+. Maximal velocities with Mn2+ is about 50% of that with Mg2+ Actinidia deliciosa
Mn2+ KM: about 0.02 mM, divalent cation required, preference for Mg2+. Maximal velocities with Mn2+ is about 50% of that with Mg2+ Actinidia deliciosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
88100
-
 x * 88100, calculated from sequence Actinidia deliciosa

Organism

Organism UniProt Comment Textmining
Actinidia deliciosa C7SHN9 cultivars ‘Chieftain’ and ‘Hayward’
-

Purification (Commentary)

Purification (Comment) Organism
-
 Actinidia deliciosa

Source Tissue

Source Tissue Comment Organism Textmining
flower expression of AdAFS1 is significantly higher in flowers than in leaf tissue. Within floral tissues, expression is highest in petals and stamens. AdAFS1 expression is low in sepals in both sexes and also in ‘Hayward’ pistils. AdAFS1 expression in ‘Hayward’ is co-ordinated with anthesis, with low levels of expression in unopened flower buds and high levels detected in open flowers. The temporal accumulation patterns of AdAFS1 mRNA is constitutive. No difference in expression between male and female flowers Actinidia deliciosa
-
leaf expression of AdAFS1 is significantly higher in flowers than in leaf tissue. AdAFS1 expression is similar in leaves of both cultivars ‘Chieftain’ and ‘Hayward’ Actinidia deliciosa
-
petal within floral tissues, expression is highest in petals and stamens Actinidia deliciosa
-
stamen within floral tissues, expression is highest in petals and stamens Actinidia deliciosa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate the product consists primarily of (E,E)-alpha-farnesene (more than 95%) with trace amounts of (Z,E)-alpha-farnesene Actinidia deliciosa (3E,6E)-alpha-farnesene + diphosphate
-
 ?
geranyl diphosphate AdAFS1 can function as a bifunctional enzyme possessing both sesquiterpene and monoterpene synthase activities. Exhibits significant monoterpene synthase activity, producing exclusively (E)-beta-ocimene. kcat/Km is about 5fold lower than kcat/Km for farnesyl diphosphate Actinidia deliciosa (E)-beta-ocimene + diphosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 88100, calculated from sequence Actinidia deliciosa

Synonyms

Synonyms Comment Organism
AdAFS1
-
 Actinidia deliciosa
alpha farnesene synthase 1
-
 Actinidia deliciosa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.03
-
 geranyl diphosphate pH 7.5 Actinidia deliciosa
0.44
-
 (2E,6E)-farnesyl diphosphate pH 7.5 Actinidia deliciosa

pI Value

Organism Comment pI Value Maximum pI Value
Actinidia deliciosa calculated from sequence
-
 5.93

Expression

Organism Comment Expression
Actinidia deliciosa expression of AdAFS1 is significantly higher in flowers than in leaf tissue. Within floral tissues, expression is highest in petals and stamens. AdAFS1 expression is low in sepals in both sexes and also in ‘Hayward’ pistils. AdAFS1 expression in ‘Hayward’ is co-ordinated with anthesis, with low levels of expression in unopened flower buds and high levels detected in open flowers. The temporal accumulation patterns of AdAFS1 mRNA is constitutive. No difference in expression between male and female flowers. AdAFS1 expression is similar in leaves of both cultivars ‘Chieftain’ and ‘Hayward’ additional information

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
11
-
 geranyl diphosphate pH 7.5 Actinidia deliciosa
51
-
 (2E,6E)-farnesyl diphosphate pH 7.5 Actinidia deliciosa