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Literature summary for 4.2.3.4 extracted from

  • Negron, L.; Patchett, M.L.; Parker, E.J.
    Expression, purification, and characterisation of dehydroquinate synthase from Pyrococcus furiosus (2011), Enzyme Res., 2011, 134893.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.3.4

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed in Escherichia coli Rosetta DE3. The enzyme is insoluble when expressed in Escherichia coli but is partially solubilised when KCl is included in the cell lysis buffer Pyrococcus furiosus

Inhibitors

Inhibitors Comment Organism Structure
EDTA inactivates the enzyme, and enzyme activity is restored by several divalent metal ions including (in order of decreasing effectiveness) Cd2+, Co2+, Zn2+, and Mn2+ Pyrococcus furiosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0037
-
 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate pH 6.8, 60°C Pyrococcus furiosus

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+ activates Pyrococcus furiosus
Co2+ activates Pyrococcus furiosus
Mn2+ activates Pyrococcus furiosus
Zn2+ activates Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37397
-
 2 * 37397, electrospray ionisation mass spectrometry Pyrococcus furiosus
72000
-
 gel filtration Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate Pyrococcus furiosus the enzyme catalyses the second step of the shikimate pathway to aromatic compounds 3-dehydroquinate + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus Q8U0A8
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate
-
 Pyrococcus furiosus 3-dehydroquinate + phosphate
-
 ?
3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate the enzyme catalyses the second step of the shikimate pathway to aromatic compounds Pyrococcus furiosus 3-dehydroquinate + phosphate
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 37397, electrospray ionisation mass spectrometry Pyrococcus furiosus

Synonyms

Synonyms Comment Organism
DHQS
-
 Pyrococcus furiosus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
90
-
 active up to Pyrococcus furiosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
 Tm-value Pyrococcus furiosus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3
-
 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate pH 6.8, 60°C Pyrococcus furiosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
-
 Pyrococcus furiosus

pH Range

pH Minimum pH Maximum Comment Organism
6.3 9.5 pH 6.3: 65% of maximal activity, pH 9.5: about 65% of maximal activity Pyrococcus furiosus

General Information

General Information Comment Organism
physiological function the enzyme catalyses the second step of the shikimate pathway to aromatic compounds Pyrococcus furiosus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
811
-
 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate pH 6.8, 60°C Pyrococcus furiosus