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Literature summary for 4.2.3.4 extracted from

  • Bender, S.; Mehdi, S.; Knowles, J.
    Dehydroquinate synthase: The role of divalent metal cations and of nicotinamide adenine dinucleotide in catalysis (1989), Biochemistry, 28, 7555-7560.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
EDTA treatment of the Co2+-holoenzyme with EDTA in the absence of substrate results in the rapid loss of catalytic activity Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00055
-
3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate at 20°C and pH 8.5 Escherichia coli
0.0006
-
3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate at 20°C and pH 6.5 Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+ reconstitution of the apoenzyme with Cd2+ restores activity to12% of the level observed with the Co2+-holoenzyme Escherichia coli
Co2+ contains 1 mol of Co2+, required for 100% activity Escherichia coli
Cu2+ reconstitution of the apoenzyme with Zn2+ restores activity to 9% of the level observed with the Co2+-holoenzyme Escherichia coli
Mn2+ reconstitution of the apoenzyme with Mn2+ restores activity to 10% of the level observed with the Co2+-holoenzyme Escherichia coli
additional information no activity in the presence of Mg2+, Ca2+, Sr2+, and Ba2+ Escherichia coli
Ni2+ reconstitution of the apoenzyme with Ni2+ restores activity to 23% of the level observed with the Co2+-holoenzyme Escherichia coli
Zn2+ reconstitution of the apoenzyme with Zn2+ restores activity to 53% of the level observed with the Co2+-holoenzyme Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli RB791
-
-
-

Storage Stability

Storage Stability Organism
4°C, free enzyme in the presence of Co2+ and NAD+, at pH 7.5 in 100 mM MOPS buffer, several months, the enzyme remains stable Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate
-
Escherichia coli 3-dehydroquinate + phosphate
-
?
3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate
-
Escherichia coli RB791 3-dehydroquinate + phosphate
-
?

Synonyms

Synonyms Comment Organism
dehydroquinate synthase
-
Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
25000
-
3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate at 20°C and pH 7.8 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NAD+ required for activity Escherichia coli