Literature summary for 4.2.3.38 extracted from

McAndrew, R.P.; Peralta-Yahya, P.P.; DeGiovanni, A.; Pereira, J.H.; Hadi, M.Z.; Keasling, J.D.; Adams, P.D.
Structure of a three-domain sesquiterpene synthase: a prospective target for advanced biofuels production (2011), Structure, 19, 1876-1884.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Abies grandis

Crystallization (Commentary)

Crystallization (Comment)	Organism
apo form and bound to five inhibitors, sitting drop vapor diffusion method, using 100 mM Tris pH 8.5, 100 mM NaCl, and 23% (w/v) PEG 3350	Abies grandis

Inhibitors

Inhibitors	Comment	Organism	Structure
alendronate	-	Abies grandis
etidronate	-	Abies grandis
farnesyl thiophosphate	-	Abies grandis
geranyl thiodiphosphate	-	Abies grandis
pamidronate	-	Abies grandis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0495	-	(2E,6E)-farnesyl diphosphate	in 25 mM Tris, 15 mM MgCl2, pH 7.5, at 30°C	Abies grandis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Abies grandis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(2E,6E)-farnesyl diphosphate	Abies grandis	-	(E)-alpha-bisabolene + diphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Abies grandis	O81086	-	-

Purification (Commentary)

Purification (Comment)	Organism
HisTrap column chromatography and Superdex 200 gel filtration	Abies grandis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(2E,6E)-farnesyl diphosphate	-	Abies grandis	(E)-alpha-bisabolene + diphosphate	-	?

Synonyms

Synonyms	Comment	Organism
apha-bisabolene synthase	-	Abies grandis
BIS	-	Abies grandis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.002	-	(2E,6E)-farnesyl diphosphate	in 25 mM Tris, 15 mM MgCl2, pH 7.5, at 30°C	Abies grandis

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.038	-	(2E,6E)-farnesyl diphosphate	in 25 mM Tris, 15 mM MgCl2, pH 7.5, at 30°C	Abies grandis

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Release 2023.1 (January 2023)