Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 5 mM, required for full activity | Streptomyces sp. | |
Tween-80 | 0.1%, required for full activity | Streptomyces sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00026 | - |
ent-copalyl diphosphate | 30°C, pH 7.0 | Streptomyces sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 1 mM, 100% of the activity with Mg2+ | Streptomyces sp. | |
Mg2+ | absolutely required, optimal concentraion 1 mM | Streptomyces sp. | |
additional information | no or very weak activity in presence of Mn2+, Ca2+, Cu2+ and Fe2+ | Streptomyces sp. | |
Ni2+ | 1 mM, 57% of the activity with Mg2+ | Streptomyces sp. | |
Zn2+ | 1 mM, 59% of the activity with Mg2+ | Streptomyces sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
gel filtration | Streptomyces sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sp. | - |
- |
- |
Streptomyces sp. KO-3988 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ent-copalyl diphosphate | - |
Streptomyces sp. | pimara-9(11),15-diene + diphosphate | - |
? | |
ent-copalyl diphosphate | - |
Streptomyces sp. KO-3988 | pimara-9(11),15-diene + diphosphate | - |
? | |
additional information | no substrates: farnesyl diphosphate, geranylgeranyl diphosphate, terpentecin diphosphate | Streptomyces sp. | ? | - |
? | |
additional information | no substrates: farnesyl diphosphate, geranylgeranyl diphosphate, terpentecin diphosphate | Streptomyces sp. KO-3988 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | x * 35000, SDS-PAGE | Streptomyces sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
0.05 M Tris, pH 7.0 | Streptomyces sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
pH 5.5, stable for at least 1 h | Streptomyces sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0014 | - |
ent-copalyl diphosphate | 30°C, pH 7.0 | Streptomyces sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Streptomyces sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | - |
Streptomyces sp. |