Protein Variants | Comment | Organism |
---|---|---|
G56A | significant increase in Hill coefficient, large decrease in turnover number | Thermus sp. |
G56S | significant increase in Hill coefficient, large decrease in turnover number | Thermus sp. |
additional information | mutant protein carrying the C-terminal tail of Escherichia coli enzyme containing an Arg residue shows elevated cooperativity in the presence of phosphate | Thermus sp. |
R97K | mutant has lost its heterotropic cooperativity, exhibits homotropic allostery, displays significantly reduced catalytic efficiency | Thermus sp. |
S55D | mutant has lost its allostery | Thermus sp. |
S55K | increase in sensitivity to phosphate | Thermus sp. |
V101A | mutant has lost its allostery | Thermus sp. |
V101I | mutant has lost its heterotropic cooperativity, exhibits homotropic allostery, displays significantly reduced catalytic efficiency | Thermus sp. |
V101S | mutant has lost its heterotropic cooperativity, exhibits homotropic allostery, displays significantly reduced catalytic efficiency | Thermus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
dihydroxyacetone phosphate | mutant S55K, Hill coefficient 1.3, pH 6.5, 60°C | Thermus sp. | |
0.21 | - |
dihydroxyacetone phosphate | mutant carrying C-terminus of Escherichia coli enzyme, Hill coefficient 0.87, pH 6.5, 60°C | Thermus sp. | |
0.41 | - |
dihydroxyacetone phosphate | mutant mutant carrying C-terminus of Escherichia coli enzyme, Hill coefficient 1.99, presence of 1.5 mM phosphate, pH 6.5, 60°C | Thermus sp. | |
0.5 | - |
dihydroxyacetone phosphate | wild-type, Hill coefficient 1, pH 6.5, 60°C | Thermus sp. | |
1.03 | - |
dihydroxyacetone phosphate | mutant V101A, Hill coefficient 1, pH 6.5, 60°C | Thermus sp. | |
1.17 | - |
dihydroxyacetone phosphate | wild-type, Hill coefficient 1.5, presence of 1.5 mM phosphate, pH 6.5, 60°C | Thermus sp. | |
1.25 | - |
dihydroxyacetone phosphate | mutant G56S, Hill coefficient 2.3, pH 6.5, 60°C | Thermus sp. | |
1.38 | - |
dihydroxyacetone phosphate | mutant S55K, Hill coefficient 1.3, presence of 1.5 mM phosphate, pH 6.5, 60°C | Thermus sp. | |
1.44 | - |
dihydroxyacetone phosphate | mutant V101I, Hill coefficient 1.44, pH 6.5, 60°C | Thermus sp. | |
1.65 | - |
dihydroxyacetone phosphate | mutant V101S, Hill coefficient 1.3, pH 6.5, 60°C | Thermus sp. | |
1.81 | - |
dihydroxyacetone phosphate | mutant V101A, Hill coefficient 1.45, presence of 1.5 mM phosphate, pH 6.5, 60°C | Thermus sp. | |
2.4 | - |
dihydroxyacetone phosphate | mutant G56A, Hill coefficient 2, pH 6.5, 60°C | Thermus sp. | |
2.7 | - |
dihydroxyacetone phosphate | mutant V101I, Hill coefficient 1.4, presence of 1.5 mM phosphate, pH 6.5, 60°C | Thermus sp. | |
2.76 | - |
dihydroxyacetone phosphate | mutant G56A, Hill coefficient 1.6, presence of 1.5 mM phosphate, pH 6.5, 60°C | Thermus sp. | |
2.76 | - |
dihydroxyacetone phosphate | mutant S55D, Hill coefficient 1, pH 6.5, 60°C | Thermus sp. | |
2.79 | - |
dihydroxyacetone phosphate | mutant R97K, Hill coefficient 1.55, pH 6.5, 60°C | Thermus sp. | |
3.27 | - |
dihydroxyacetone phosphate | mutant S55D, Hill coefficient 1.2, presence of 1.5 mM phosphate, pH 6.5, 60°C | Thermus sp. | |
3.5 | - |
dihydroxyacetone phosphate | mutant G56S, Hill coefficient 2.8, presence of 1.5 mM phosphate, pH 6.5, 60°C | Thermus sp. | |
5.03 | - |
dihydroxyacetone phosphate | mutant V101S, Hill coefficient 1.22, presence of 1.5 mM phosphate, pH 6.5, 60°C | Thermus sp. | |
6.3 | - |
dihydroxyacetone phosphate | mutant R97K, Hill coefficient 1.55, presence of 1.5 mM phosphate, pH 6.5, 60°C | Thermus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
14300 | - |
2 * 14300, calculated, minor form. The main form is the hexamer | Thermus sp. |
14300 | - |
6 * 14300, calculated, main form plus some dimer | Thermus sp. |
31000 | - |
GEMMA analysis, minor peak | Thermus sp. |
89000 | - |
GEMMA analysis, main peak | Thermus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus sp. | B3VH91 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydroxyacetone phosphate | - |
Thermus sp. | methylglyoxal + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 14300, calculated, minor form. The main form is the hexamer | Thermus sp. |
hexamer | 6 * 14300, calculated, main form plus some dimer | Thermus sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00003 | - |
dihydroxyacetone phosphate | mutant G56S, Hill coefficient 2.8, presence of 1.5 mM phosphate, pH 6.5, 60°C | Thermus sp. | |
0.00005 | - |
dihydroxyacetone phosphate | mutant G56S, Hill coefficient 2.3, pH 6.5, 60°C | Thermus sp. | |
0.0001 | - |
dihydroxyacetone phosphate | mutant G56A, Hill coefficient 1.6, presence of 1.5 mM phosphate, pH 6.5, 60°C | Thermus sp. | |
0.00013 | - |
dihydroxyacetone phosphate | mutant G56A, Hill coefficient 2, pH 6.5, 60°C | Thermus sp. | |
0.37 | - |
dihydroxyacetone phosphate | mutant V101I, Hill coefficient 1.4, presence of 1.5 mM phosphate, pH 6.5, 60°C | Thermus sp. | |
0.55 | - |
dihydroxyacetone phosphate | mutant S55K, Hill coefficient 1.3, presence of 1.5 mM phosphate, pH 6.5, 60°C | Thermus sp. | |
0.62 | - |
dihydroxyacetone phosphate | mutant R97K, Hill coefficient 1.55, presence of 1.5 mM phosphate, pH 6.5, 60°C | Thermus sp. | |
0.75 | - |
dihydroxyacetone phosphate | mutant R97K, Hill coefficient 1.55, pH 6.5, 60°C | Thermus sp. | |
0.75 | - |
dihydroxyacetone phosphate | mutant V101I, Hill coefficient 1.44, pH 6.5, 60°C | Thermus sp. | |
1 | - |
dihydroxyacetone phosphate | mutant S55K, Hill coefficient 1.3, pH 6.5, 60°C | Thermus sp. | |
1.1 | - |
dihydroxyacetone phosphate | mutant V101S, Hill coefficient 1.22, presence of 1.5 mM phosphate, pH 6.5, 60°C | Thermus sp. | |
1.4 | - |
dihydroxyacetone phosphate | mutant V101S, Hill coefficient 1.3, pH 6.5, 60°C | Thermus sp. | |
76 | - |
dihydroxyacetone phosphate | mutant mutant carrying C-terminus of Escherichia coli enzyme, Hill coefficient 1.99, presence of 1.5 mM phosphate, pH 6.5, 60°C | Thermus sp. | |
100 | - |
dihydroxyacetone phosphate | mutant carrying C-terminus of Escherichia coli enzyme, Hill coefficient 0.87, pH 6.5, 60°C | Thermus sp. | |
220 | - |
dihydroxyacetone phosphate | wild-type, Hill coefficient 1.5, presence of 1.5 mM phosphate, pH 6.5, 60°C | Thermus sp. | |
240 | - |
dihydroxyacetone phosphate | mutant V101A, Hill coefficient 1.45, presence of 1.5 mM phosphate, pH 6.5, 60°C | Thermus sp. | |
260 | - |
dihydroxyacetone phosphate | mutant S55D, Hill coefficient 1.2, presence of 1.5 mM phosphate, pH 6.5, 60°C | Thermus sp. | |
300 | - |
dihydroxyacetone phosphate | mutant S55D, Hill coefficient 1, pH 6.5, 60°C | Thermus sp. | |
327 | - |
dihydroxyacetone phosphate | wild-type, Hill coefficient 1, pH 6.5, 60°C | Thermus sp. | |
338 | - |
dihydroxyacetone phosphate | mutant V101A, Hill coefficient 1, pH 6.5, 60°C | Thermus sp. |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00004 | - |
dihydroxyacetone phosphate | mutant G56A, Hill coefficient 1.6, presence of 1.5 mM phosphate, pH 6.5, 60°C | Thermus sp. | |
0.00004 | - |
dihydroxyacetone phosphate | mutant G56S, Hill coefficient 2.3, pH 6.5, 60°C | Thermus sp. | |
0.00005 | - |
dihydroxyacetone phosphate | mutant G56A, Hill coefficient 2, pH 6.5, 60°C | Thermus sp. | |
0.00009 | - |
dihydroxyacetone phosphate | mutant G56S, Hill coefficient 2.8, presence of 1.5 mM phosphate, pH 6.5, 60°C | Thermus sp. | |
0.1 | - |
dihydroxyacetone phosphate | mutant R97K, Hill coefficient 1.55, presence of 1.5 mM phosphate, pH 6.5, 60°C | Thermus sp. | |
0.14 | - |
dihydroxyacetone phosphate | mutant V101I, Hill coefficient 1.4, presence of 1.5 mM phosphate, pH 6.5, 60°C | Thermus sp. | |
0.22 | - |
dihydroxyacetone phosphate | mutant V101S, Hill coefficient 1.22, presence of 1.5 mM phosphate, pH 6.5, 60°C | Thermus sp. | |
0.27 | - |
dihydroxyacetone phosphate | mutant R97K, Hill coefficient 1.55, pH 6.5, 60°C | Thermus sp. | |
0.4 | - |
dihydroxyacetone phosphate | mutant S55K, Hill coefficient 1.3, presence of 1.5 mM phosphate, pH 6.5, 60°C | Thermus sp. | |
0.52 | - |
dihydroxyacetone phosphate | mutant V101I, Hill coefficient 1.44, pH 6.5, 60°C | Thermus sp. | |
0.86 | - |
dihydroxyacetone phosphate | mutant V101S, Hill coefficient 1.3, pH 6.5, 60°C | Thermus sp. | |
12 | - |
dihydroxyacetone phosphate | mutant S55K, Hill coefficient 1.3, pH 6.5, 60°C | Thermus sp. | |
79 | - |
dihydroxyacetone phosphate | mutant S55D, Hill coefficient 1.2, presence of 1.5 mM phosphate, pH 6.5, 60°C | Thermus sp. | |
110 | - |
dihydroxyacetone phosphate | mutant S55D, Hill coefficient 1, pH 6.5, 60°C | Thermus sp. | |
130 | - |
dihydroxyacetone phosphate | mutant V101A, Hill coefficient 1.45, presence of 1.5 mM phosphate, pH 6.5, 60°C | Thermus sp. | |
180 | - |
dihydroxyacetone phosphate | mutant mutant carrying C-terminus of Escherichia coli enzyme, Hill coefficient 1.99, presence of 1.5 mM phosphate, pH 6.5, 60°C | Thermus sp. | |
190 | - |
dihydroxyacetone phosphate | wild-type, Hill coefficient 1.5, presence of 1.5 mM phosphate, pH 6.5, 60°C | Thermus sp. | |
330 | - |
dihydroxyacetone phosphate | mutant V101A, Hill coefficient 1, pH 6.5, 60°C | Thermus sp. | |
490 | - |
dihydroxyacetone phosphate | mutant carrying C-terminus of Escherichia coli enzyme, Hill coefficient 0.87, pH 6.5, 60°C | Thermus sp. | |
653 | - |
dihydroxyacetone phosphate | wild-type, Hill coefficient 1, pH 6.5, 60°C | Thermus sp. |