Protein Variants | Comment | Organism |
---|---|---|
DELTAR97 | strong decrease in activity, increase in Hill coefficient from 1 of wild-type to 3.3 | Thermus sp. |
R97E | strong decrease in activity, increase in Hill coefficient from 1 of wild-type to 3.4 | Thermus sp. |
R97K | strong decrease in activity, increase in Hill coefficient from 1 of wild-type to 1.5 | Thermus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
dihydroxyacetone phosphate | wild-type, Hill coefficient 1, pH 6.5, 60°C | Thermus sp. | |
0.8 | - |
dihydroxyacetone phosphate | wild-type, Hill coefficient 1.2, presence of 1 mM phosphate, pH 6.5, 60°C | Thermus sp. | |
1.5 | - |
dihydroxyacetone phosphate | mutant R97E, Hill coefficient 3.4, pH 6.5, 60°C | Thermus sp. | |
1.6 | - |
dihydroxyacetone phosphate | mutant R97E, Hill coefficient 3.7, presence of 1 mM phosphate, pH 6.5, 60°C | Thermus sp. | |
1.7 | - |
dihydroxyacetone phosphate | mutant DELTAR97, Hill coefficient 3.3, pH 6.5, 60°C | Thermus sp. | |
1.7 | - |
dihydroxyacetone phosphate | mutant DELTAR97, Hill coefficient 5.2, presence of 1 mM phosphate, pH 6.5, 60°C | Thermus sp. | |
2.8 | - |
dihydroxyacetone phosphate | mutant R97K, Hill coefficient 1.5, pH 6.5, 60°C | Thermus sp. | |
3.2 | - |
dihydroxyacetone phosphate | mutant R97K, Hill coefficient 1.5, presence of 1 mM phosphate, pH 6.5, 60°C | Thermus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus sp. | B3VH91 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydroxyacetone phosphate | - |
Thermus sp. | methylglyoxal + phosphate | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
10 min, wild-type retains 84 of initial activity, mutant R97E shows complete loss of activity | Thermus sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00025 | - |
dihydroxyacetone phosphate | mutant DELTAR97, Hill coefficient 5.2, presence of 1 mM phosphate, pH 6.5, 60°C | Thermus sp. | |
0.00025 | - |
dihydroxyacetone phosphate | mutant R97E, Hill coefficient 3.7, presence of 1 mM phosphate, pH 6.5, 60°C | Thermus sp. | |
0.0004 | - |
dihydroxyacetone phosphate | mutant R97E, Hill coefficient 3.4, pH 6.5, 60°C | Thermus sp. | |
0.001 | - |
dihydroxyacetone phosphate | mutant DELTAR97, Hill coefficient 3.3, pH 6.5, 60°C | Thermus sp. | |
0.5 | - |
dihydroxyacetone phosphate | mutant R97K, Hill coefficient 1.5, presence of 1 mM phosphate, pH 6.5, 60°C | Thermus sp. | |
0.7 | - |
dihydroxyacetone phosphate | mutant R97K, Hill coefficient 1.5, pH 6.5, 60°C | Thermus sp. | |
233 | - |
dihydroxyacetone phosphate | wild-type, Hill coefficient 1.2, presence of 1 mM phosphate, pH 6.5, 60°C | Thermus sp. | |
326 | - |
dihydroxyacetone phosphate | wild-type, Hill coefficient 1, pH 6.5, 60°C | Thermus sp. |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00015 | - |
dihydroxyacetone phosphate | mutant DELTAR97, Hill coefficient 5.2, presence of 1 mM phosphate, pH 6.5, 60°C | Thermus sp. | |
0.00016 | - |
dihydroxyacetone phosphate | mutant R97E, Hill coefficient 3.7, presence of 1 mM phosphate, pH 6.5, 60°C | Thermus sp. | |
0.0003 | - |
dihydroxyacetone phosphate | mutant R97E, Hill coefficient 3.4, pH 6.5, 60°C | Thermus sp. | |
0.0006 | - |
dihydroxyacetone phosphate | mutant DELTAR97, Hill coefficient 3.3, pH 6.5, 60°C | Thermus sp. | |
0.2 | - |
dihydroxyacetone phosphate | mutant R97K, Hill coefficient 1.5, presence of 1 mM phosphate, pH 6.5, 60°C | Thermus sp. | |
0.3 | - |
dihydroxyacetone phosphate | mutant R97K, Hill coefficient 1.5, pH 6.5, 60°C | Thermus sp. | |
288 | - |
dihydroxyacetone phosphate | wild-type, Hill coefficient 1.2, presence of 1 mM phosphate, pH 6.5, 60°C | Thermus sp. | |
653 | - |
dihydroxyacetone phosphate | wild-type, Hill coefficient 1, pH 6.5, 60°C | Thermus sp. |