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Literature summary for 4.2.3.3 extracted from
- Methylglyoxal synthase (1975), Methods Enzymol., 41B, 502-508.
General Stability
| General Stability | Organism |
|---|---|
| removal of phosphate from the purified enzyme leads to rapid loss of activity, which is more pronounced at 0°C than at 22°C. Dihydroxyacetone phosphate and bovine serum albumin overcome the inactivation to some extent | Pelomonas saccharophila |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3-phosphoglycerate | - |
Pelomonas saccharophila |  |
| diphosphate | - |
Pelomonas saccharophila | |
| phosphoenolpyruvate | - |
Pelomonas saccharophila | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.09 | - |
dihydroxyacetone phosphate | - |
Pelomonas saccharophila | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 67000 | - |
gel filtration | Pelomonas saccharophila |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dihydroxyacetone phosphate | Pelomonas saccharophila | constitutive enzyme, may be involved in by-pass sequence for part of the Embden-Meyerhof glycolytic pathway | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aeromonas caviae | - |
- |
- |
| Clostridium pasteurianum | - |
- |
- |
| Clostridium tetanomorphum | - |
- |
- |
| Obesumbacterium proteus | - |
- |
- |
| Pelomonas saccharophila | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Pelomonas saccharophila |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dihydroxyacetone phosphate | - |
Clostridium pasteurianum | methylglyoxal + phosphate | - |
? | |
| dihydroxyacetone phosphate | - |
Aeromonas caviae | methylglyoxal + phosphate | - |
? | |
| dihydroxyacetone phosphate | - |
Pelomonas saccharophila | methylglyoxal + phosphate | - |
ir | |
| dihydroxyacetone phosphate | - |
Clostridium tetanomorphum | methylglyoxal + phosphate | - |
? | |
| dihydroxyacetone phosphate | - |
Obesumbacterium proteus | methylglyoxal + phosphate | - |
? | |
| dihydroxyacetone phosphate | constitutive enzyme, may be involved in by-pass sequence for part of the Embden-Meyerhof glycolytic pathway | Pelomonas saccharophila | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.2 | - |
- |
Pelomonas saccharophila |
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Release 2023.1 (January 2023)
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