Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-ethylmaleimide | pre-incubation of the partially-purified enzyme with 5 mM N-ethylmaleimide results in 66% inhibition of activity | Campylopus introflexus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.37 | - |
dimethylallyl diphosphate | at pH 8.6 and 40°C | Campylopus introflexus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, 50-60% activity at 15 mM, the enzyme utilizes Mn2+ preferentially over Mg2+ | Campylopus introflexus | |
Mn2+ | required, 100% activity at 15 mM, the enzyme utilizes Mn2+ preferentially over Mg2+ | Campylopus introflexus | |
additional information | not activated by Zn2+ or Ca2+ | Campylopus introflexus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate | Campylopus introflexus | - |
isoprene + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Campylopus introflexus | - |
heath star moss | - |
Purification (Comment) | Organism |
---|---|
DEAE column chromatography, and Superdex 200 gel filtration | Campylopus introflexus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
shoot | - |
Campylopus introflexus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate | - |
Campylopus introflexus | isoprene + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ISPS | - |
Campylopus introflexus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Campylopus introflexus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.6 | - |
- |
Campylopus introflexus |