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Literature summary for 4.2.3.27 extracted from

  • Zhou, C.; Li, Z.; Wiberley-Bradford, A.E.; Weise, S.E.; Sharkey, T.D.
    Isopentenyl diphosphate and dimethylallyl diphosphate/isopentenyl diphosphate ratio measured with recombinant isopentenyl diphosphate isomerase and isoprene synthase (2013), Anal. Biochem., 440, 130-136.
    View publication on PubMed

Application

Application Comment Organism
analysis development of a method to measure the isopentenyl diphosphate levels and the ratio of dimethyallyl diphosphate/isopentenyl diphosphate in bacteria and plant tissues. The amount of dimethyallyl diphosphate is measured by conversion to isoprene using isoprene synthase IspS, and the total amount of dimethyallyl diphosphate plus isopentenyl diphosphate is measured by including isopentenyldiphosphate isomerase IDI in the assay mixture. The in vitro equilibrium ratio of dimethylallyl diphosphate/isopentenyl diphosphate is 2.11:1. Isopentenyl diphosphate and dimethylallayl diphosphate pools are significantly increased in Escherichia coli transformed with methylerythritol 4-phosphate pathway genes; the ratio dimethyallyl diphosphate/isopentenyl diphosphate is 3.85. An Escherichia coli strain transformed with IspS but no additional IDI has a lower dimethylallyl diphosphate level and a dimethyallyl diphosphate/isopentenyl diphosphate ratio of 1.05 Eucalyptus sp.

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Eucalyptus sp.

Organism

Organism UniProt Comment Textmining
Eucalyptus sp.
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