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Literature summary for 4.2.3.27 extracted from

  • Rasulov, B.; Hueve, K.; Bichele, I.; Laisk, A.; Niinemets, U.
    Temperature response of isoprene emission in vivo reflects a combined effect of substrate limitations and isoprene synthase activity: a kinetic analysis (2010), Plant Physiol., 154, 1558-1570.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
analysis method for measuring isoprene emission based on postillumination isoprene release after rapid temperature transient, and application to determine the rate constant of isoprene synthase, the pool size of its substrate dimethylallyldiphosphate, and to separate the component processes of the temperature dependence of isoprene emission Populus sp.
environmental protection analysis of in vivo isoprene emission. The in vivo rate constant of IspS obeys the Arrhenius law, with an activation energy of 42.8 kJ per mol. Steady-state isoprene emission has a significantly lower temperature optimum than IspS and higher activation energy. The reversible temperature-dependent decrease in the rate of isoprene emission between 35°C and 44°C is caused by decreases in dimethylallyl diphosphate concentration, possibly reflecting reduced pools of energetic metabolites generated in photosynthesis, particularly of ATP. Strong control of isoprene temperature responses by the dimethylallyl diphosphate pool implies that transient temperature responses under fluctuating conditions in the field are driven by initial dimethylallyl diphosphate pool size as well as temperature-dependent modifications in dimethylallyl diphosphate pool size during temperature transients Populus sp.

Organism

Organism UniProt Comment Textmining
Populus sp.
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hybrid, Populus tremula x Populus tremuloides
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Source Tissue

Source Tissue Comment Organism Textmining
leaf
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Populus sp.
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Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
additional information
-
the in vivo rate constant of IspS obeys the Arrhenius law, with an activation energy of 42.8 kJ per mol Populus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25 45 stable within Populus sp.