Application | Comment | Organism |
---|---|---|
analysis | method for measuring isoprene emission based on postillumination isoprene release after rapid temperature transient, and application to determine the rate constant of isoprene synthase, the pool size of its substrate dimethylallyldiphosphate, and to separate the component processes of the temperature dependence of isoprene emission | Populus sp. |
environmental protection | analysis of in vivo isoprene emission. The in vivo rate constant of IspS obeys the Arrhenius law, with an activation energy of 42.8 kJ per mol. Steady-state isoprene emission has a significantly lower temperature optimum than IspS and higher activation energy. The reversible temperature-dependent decrease in the rate of isoprene emission between 35°C and 44°C is caused by decreases in dimethylallyl diphosphate concentration, possibly reflecting reduced pools of energetic metabolites generated in photosynthesis, particularly of ATP. Strong control of isoprene temperature responses by the dimethylallyl diphosphate pool implies that transient temperature responses under fluctuating conditions in the field are driven by initial dimethylallyl diphosphate pool size as well as temperature-dependent modifications in dimethylallyl diphosphate pool size during temperature transients | Populus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Populus sp. | - |
hybrid, Populus tremula x Populus tremuloides | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Populus sp. | - |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
the in vivo rate constant of IspS obeys the Arrhenius law, with an activation energy of 42.8 kJ per mol | Populus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 45 | stable within | Populus sp. |