Crystallization (Comment) | Organism |
---|---|
X-ray crystal structure of recombinant ISPS at 2.7 A resolution, and the structure of its complex with three Mg2+ and theunreactive substrate analogue dimethylallyl-S-thiolodiphosphate determined at 2.8 A resolution. Analysis suggests that the generation of isoprene from substrate dimethylallyl diphosphate occurs via a syn-periplanar elimination mechanism in which the diphosphate-leaving group serves as a general base | Populus tremula x Populus alba |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | the metal binding motifs of ISPS interact with a trinuclear magnesium cluster in the complex with substrate dimethylallyl diphosphate. While Mg2+A is bound with full occupancy, the occupancies of Mg2+B and Mg2+C are each estimated to be 0.75. Full occupancy binding of Mg2+B and Mg2+C may accompany the transition to a closed active-site conformation characterized by optimal metal coordination interactions | Populus tremula x Populus alba |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Populus tremula x Populus alba | Q9AR86 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
dimethylallyl diphosphate = isoprene + diphosphate | the generation of isoprene from substrate dimethylallyl diphosphate occurs via a syn-periplanar elimination mechanism in which the diphosphate-leaving group serves as a general base | Populus tremula x Populus alba |