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Literature summary for 4.2.3.27 extracted from

  • Koeksal, M.; Zimmer, I.; Schnitzler, J.P.; Christianson, D.W.
    Structure of isoprene synthase illuminates the chemical mechanism of teragram atmospheric carbon emission (2010), J. Mol. Biol., 402, 363-373.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
X-ray crystal structure of recombinant ISPS at 2.7 A resolution, and the structure of its complex with three Mg2+ and theunreactive substrate analogue dimethylallyl-S-thiolodiphosphate determined at 2.8 A resolution. Analysis suggests that the generation of isoprene from substrate dimethylallyl diphosphate occurs via a syn-periplanar elimination mechanism in which the diphosphate-leaving group serves as a general base Populus tremula x Populus alba

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ the metal binding motifs of ISPS interact with a trinuclear magnesium cluster in the complex with substrate dimethylallyl diphosphate. While Mg2+A is bound with full occupancy, the occupancies of Mg2+B and Mg2+C are each estimated to be 0.75. Full occupancy binding of Mg2+B and Mg2+C may accompany the transition to a closed active-site conformation characterized by optimal metal coordination interactions Populus tremula x Populus alba

Organism

Organism UniProt Comment Textmining
Populus tremula x Populus alba Q9AR86
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Reaction

Reaction Comment Organism Reaction ID
dimethylallyl diphosphate = isoprene + diphosphate the generation of isoprene from substrate dimethylallyl diphosphate occurs via a syn-periplanar elimination mechanism in which the diphosphate-leaving group serves as a general base Populus tremula x Populus alba