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Literature summary for 4.2.3.27 extracted from

  • Zhao, Y.; Yang, J.; Qin, B.; Li, Y.; Sun, Y.; Su, S.; Xian, M.
    Biosynthesis of isoprene in Escherichia coli via methylerythritol phosphate (MEP) pathway (2011), Appl. Microbiol. Biotechnol., 90, 1915-1922.
    View publication on PubMed

Application

Application Comment Organism
synthesis construction of a synthetic pathway of isoprene in Escherichia coli by introducing an isoprene synthase gene from Populus nigra and overexpression of the native or Bacillus subtilis 1-deoxy-D-xylulose-5-phosphate synthase gene and 1-deoxy-D-xylulose-5-phosphate reductoisomerase gene. Transformants expressing ispS alone accumulate around 94 mg isoprene per liter of broth.Transformants expressing ispS plus the native 1-deoxy-D-xylulose-5-phosphate synthase gene and 1-deoxy-D-xylulose-5-phosphate reductoisomerase gene produce 160 mg isoprene per liter. Transformants expressing ispS plus Bacillus subtilis 1-deoxy-D-xylulose-5-phosphate synthase gene and 1-deoxy-D-xylulose-5-phosphate reductoisomerase gene produce 314 mg isoprene per liter Populus nigra

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Populus nigra

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68000
-
x * 68000, SDS-PAGE Populus nigra

Organism

Organism UniProt Comment Textmining
Populus nigra A0PFK2
-
-

Subunits

Subunits Comment Organism
? x * 68000, SDS-PAGE Populus nigra