Application | Comment | Organism |
---|---|---|
synthesis | construction of a synthetic pathway of isoprene in Escherichia coli by introducing an isoprene synthase gene from Populus nigra and overexpression of the native or Bacillus subtilis 1-deoxy-D-xylulose-5-phosphate synthase gene and 1-deoxy-D-xylulose-5-phosphate reductoisomerase gene. Transformants expressing ispS alone accumulate around 94 mg isoprene per liter of broth.Transformants expressing ispS plus the native 1-deoxy-D-xylulose-5-phosphate synthase gene and 1-deoxy-D-xylulose-5-phosphate reductoisomerase gene produce 160 mg isoprene per liter. Transformants expressing ispS plus Bacillus subtilis 1-deoxy-D-xylulose-5-phosphate synthase gene and 1-deoxy-D-xylulose-5-phosphate reductoisomerase gene produce 314 mg isoprene per liter | Populus nigra |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Populus nigra |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
68000 | - |
x * 68000, SDS-PAGE | Populus nigra |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Populus nigra | A0PFK2 | - |
- |
Subunits | Comment | Organism |
---|---|---|
? | x * 68000, SDS-PAGE | Populus nigra |