Cloned (Comment) | Organism |
---|---|
gene ispS, DNA and amino acid sequence determination and analysis, sequence comparisons, expression of His-tagged enzyme with or without leader sequence in Escherichia coli strain TG1 | Populus tremula x Populus alba |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | the enzyme contains a plastidial leader sequence | Populus tremula x Populus alba | 9507 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | dependent on, the enzyme contains the Mg2+-binding DDXXD motif, typical for monoterpene synthases | Populus tremula x Populus alba | |
Mn2+ | can substitute for Mg2+ | Populus tremula x Populus alba |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate | Populus tremula x Populus alba | - |
isoprene + diphosphate | isoprene is a volatile product emmitted to the atmosphere | ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Populus tremula x Populus alba | Q9AR86 | precursor | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the enzyme contains a plastidial leader sequence | Populus tremula x Populus alba |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain TG1 | Populus tremula x Populus alba |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.012 | 0.156 | purified recombinant enzyme with or without leader sequence | Populus tremula x Populus alba |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate | - |
Populus tremula x Populus alba | isoprene + diphosphate | isoprene is a volatile product emmitted to the atmosphere | ir | |
dimethylallyl diphosphate | - |
Populus tremula x Populus alba | isoprene + diphosphate | i.e. 2-methyl-1,3-butadiene | ir |
Subunits | Comment | Organism |
---|---|---|
More | the enzyme contains the Mg2+-binding DDXXD motif, typical for monoterpene synthases | Populus tremula x Populus alba |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
assay at | Populus tremula x Populus alba |