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Literature summary for 4.2.3.27 extracted from

  • Miller, B.; Oschinski, C.; Zimmer, W.
    First isolation of an isoprene synthase gene from poplar and successful expression of the gene in Escherichia coli (2001), Planta, 213, 483-487.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene ispS, DNA and amino acid sequence determination and analysis, sequence comparisons, expression of His-tagged enzyme with or without leader sequence in Escherichia coli strain TG1 Populus tremula x Populus alba

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast the enzyme contains a plastidial leader sequence Populus tremula x Populus alba 9507
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ dependent on, the enzyme contains the Mg2+-binding DDXXD motif, typical for monoterpene synthases Populus tremula x Populus alba
Mn2+ can substitute for Mg2+ Populus tremula x Populus alba

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dimethylallyl diphosphate Populus tremula x Populus alba
-
isoprene + diphosphate isoprene is a volatile product emmitted to the atmosphere ir

Organism

Organism UniProt Comment Textmining
Populus tremula x Populus alba Q9AR86 precursor
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification the enzyme contains a plastidial leader sequence Populus tremula x Populus alba

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain TG1 Populus tremula x Populus alba

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.012 0.156 purified recombinant enzyme with or without leader sequence Populus tremula x Populus alba

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dimethylallyl diphosphate
-
Populus tremula x Populus alba isoprene + diphosphate isoprene is a volatile product emmitted to the atmosphere ir
dimethylallyl diphosphate
-
Populus tremula x Populus alba isoprene + diphosphate i.e. 2-methyl-1,3-butadiene ir

Subunits

Subunits Comment Organism
More the enzyme contains the Mg2+-binding DDXXD motif, typical for monoterpene synthases Populus tremula x Populus alba

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
assay at Populus tremula x Populus alba