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Literature summary for 4.2.3.27 extracted from

  • Wildermuth, M.C.; Fall, R.
    Light-dependent isoprene emission (characterization of a thylakoid-bound isoprene synthase in Salix discolor chloroplasts) (1996), Plant Physiol., 112, 171-182.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information light-dependent regulation of the thylakoid membrane-bound enzyme, overview Salix discolor

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
8
-
dimethylallyl diphosphate pH 7.8 Salix discolor

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
Salix discolor 9507
-
additional information study of subcellular localization, overview Salix discolor
-
-
soluble soluble enzyme form Salix discolor
-
-
thylakoid membrane tightly membrane-bound enzyme form with a stromal facing, accessible to trypsin treatment Salix discolor 42651
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ dependent on, optimal at 10-15 mM, activity profile at 0-25 mM MgCl2 Salix discolor
Mn2+ can substitute for Mg2+ Salix discolor
additional information no effect by Ca2+ Salix discolor

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dimethylallyl diphosphate Salix discolor light-dependent regulation of the thylakoid membrane-bound enzyme, overview isoprene + diphosphate isoprene is a volatile product emmitted to the atmosphere ir

Organism

Organism UniProt Comment Textmining
Salix discolor
-
soluble and membrane-bound isozymes
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Salix discolor
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
activity in subcellular fractions during partial purification, overview Salix discolor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dimethylallyl diphosphate
-
Salix discolor isoprene + diphosphate i.e. 2-methyl-1,3-butadiene ir
dimethylallyl diphosphate light-dependent regulation of the thylakoid membrane-bound enzyme, overview Salix discolor isoprene + diphosphate isoprene is a volatile product emmitted to the atmosphere ir

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
-
Salix discolor