Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is positively regulated by light | Mucuna sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | stimulates at up to 2 mM, inhibits at 2-30 mM | Mucuna sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | stimulates at up to 2 mM, inhibits at 2-30 mM | Mucuna sp. | |
Mg2+ | dependent on, best at 15 mM, 20fold higher activity compared to Mn2+ | Mucuna sp. | |
Mn2+ | can partially substitute for Mg2+, 20fold lower activity compared to Mg2+ | Mucuna sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate | Mucuna sp. | - |
isoprene + diphosphate | isoprene is emmitted to the atmosphere | ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mucuna sp. | - |
velvet bean | - |
Purification (Comment) | Organism |
---|---|
native enzyme partially by ammonium sulfate fractionation and anion exchange chromatography | Mucuna sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | activity during leaf development, highest activity an isoprene emission in about 14-days-old leaves, overview | Mucuna sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activity and isoprene emission during leaf development, overview | Mucuna sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate | - |
Mucuna sp. | isoprene + diphosphate | isoprene is emmitted to the atmosphere | ir | |
dimethylallyl diphosphate | - |
Mucuna sp. | isoprene + diphosphate | i.e. 2-methyl-1,3-butadiene | ir |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Mucuna sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | 8.5 | - |
Mucuna sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.4 | 11.2 | - |
Mucuna sp. |