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Literature summary for 4.2.3.27 extracted from

  • Kuzma, J.; Fall, R.
    Leaf isoprene emission rate is dependent on leaf development and the level of isoprene synthase (1993), Plant Physiol., 101, 435-440.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information the enzyme is positively regulated by light Mucuna sp.

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ stimulates at up to 2 mM, inhibits at 2-30 mM Mucuna sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ stimulates at up to 2 mM, inhibits at 2-30 mM Mucuna sp.
Mg2+ dependent on, best at 15 mM, 20fold higher activity compared to Mn2+ Mucuna sp.
Mn2+ can partially substitute for Mg2+, 20fold lower activity compared to Mg2+ Mucuna sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dimethylallyl diphosphate Mucuna sp.
-
isoprene + diphosphate isoprene is emmitted to the atmosphere ir

Organism

Organism UniProt Comment Textmining
Mucuna sp.
-
velvet bean
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme partially by ammonium sulfate fractionation and anion exchange chromatography Mucuna sp.

Source Tissue

Source Tissue Comment Organism Textmining
leaf activity during leaf development, highest activity an isoprene emission in about 14-days-old leaves, overview Mucuna sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
activity and isoprene emission during leaf development, overview Mucuna sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dimethylallyl diphosphate
-
Mucuna sp. isoprene + diphosphate isoprene is emmitted to the atmosphere ir
dimethylallyl diphosphate
-
Mucuna sp. isoprene + diphosphate i.e. 2-methyl-1,3-butadiene ir

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Mucuna sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8 8.5
-
Mucuna sp.

pH Range

pH Minimum pH Maximum Comment Organism
5.4 11.2
-
Mucuna sp.