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Literature summary for 4.2.3.27 extracted from

  • Sasaki, K.; Ohara, K.; Yazaki, K.
    Gene expression and characterization of isoprene synthase from Populus alba (2005), FEBS Lett., 579, 2514-2518.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information the enzyme is induced by heat stress and continuous light irradiation Populus alba

Cloned(Commentary)

Cloned (Comment) Organism
gene ispS, DNA and amino acid sequence determination and analysis, expression in Escherichia coli, expression as GFP-fusion protein in onion peels and tobacco leaves, transformations by particle bombardement Populus alba

Inhibitors

Inhibitors Comment Organism Structure
additional information enzyme expression is highly reduced in the dark Populus alba

Localization

Localization Comment Organism GeneOntology No. Textmining
plastid the enzyme contains a plastidial targeting sequence Populus alba 9536
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activates, 23% of the activity with Mg2+ Populus alba
Fe2+ 2% of the activity with Mg2+ Populus alba
Mg2+ activates at 20 mM, best divalent cation Populus alba
Mn2+ activates, 34% of the activity with Mg2+ Populus alba
additional information the enzyme requires divalent cations, no activity with Cu2+ and Ca2+ Populus alba
Ni2+ 2% of the activity with Mg2+ Populus alba
Zn2+ 3% of the activity with Mg2+ Populus alba

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dimethylallyl diphosphate Populus alba
-
isoprene + diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Populus alba Q50L36 gene ispS; gene ispS
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification the enzyme contains a plastidial targeting sequence Populus alba

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli Populus alba

Source Tissue

Source Tissue Comment Organism Textmining
cell culture from leaves Populus alba
-
leaf
-
Populus alba
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00039
-
leaf cell culture Populus alba

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dimethylallyl diphosphate
-
Populus alba isoprene + diphosphate
-
?
dimethylallyl diphosphate the enzyme is absolutely specific for dimethylally diphosphate Populus alba isoprene + diphosphate product identification ?
additional information substrate specifcity, no enzymatic activity with isoprenyl diphosphate or geranyl diphosphate as substrates, overview Populus alba ?
-
?

Subunits

Subunits Comment Organism
More the enzyme contains the aspartate-rich motif DDxxD Populus alba

Synonyms

Synonyms Comment Organism
ISPS
-
Populus alba

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
recombinant enzyme Populus alba

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 50 about half-maximal activity at 25°C and 50°C, temperature profile Populus alba

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
recombinant enzyme Populus alba

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9.5 activity range Populus alba