Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is induced by heat stress and continuous light irradiation | Populus alba |
Cloned (Comment) | Organism |
---|---|
gene ispS, DNA and amino acid sequence determination and analysis, expression in Escherichia coli, expression as GFP-fusion protein in onion peels and tobacco leaves, transformations by particle bombardement | Populus alba |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme expression is highly reduced in the dark | Populus alba |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
plastid | the enzyme contains a plastidial targeting sequence | Populus alba | 9536 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activates, 23% of the activity with Mg2+ | Populus alba | |
Fe2+ | 2% of the activity with Mg2+ | Populus alba | |
Mg2+ | activates at 20 mM, best divalent cation | Populus alba | |
Mn2+ | activates, 34% of the activity with Mg2+ | Populus alba | |
additional information | the enzyme requires divalent cations, no activity with Cu2+ and Ca2+ | Populus alba | |
Ni2+ | 2% of the activity with Mg2+ | Populus alba | |
Zn2+ | 3% of the activity with Mg2+ | Populus alba |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate | Populus alba | - |
isoprene + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Populus alba | Q50L36 | gene ispS; gene ispS | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the enzyme contains a plastidial targeting sequence | Populus alba |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli | Populus alba |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | from leaves | Populus alba | - |
leaf | - |
Populus alba | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00039 | - |
leaf cell culture | Populus alba |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate | - |
Populus alba | isoprene + diphosphate | - |
? | |
dimethylallyl diphosphate | the enzyme is absolutely specific for dimethylally diphosphate | Populus alba | isoprene + diphosphate | product identification | ? | |
additional information | substrate specifcity, no enzymatic activity with isoprenyl diphosphate or geranyl diphosphate as substrates, overview | Populus alba | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the enzyme contains the aspartate-rich motif DDxxD | Populus alba |
Synonyms | Comment | Organism |
---|---|---|
ISPS | - |
Populus alba |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
recombinant enzyme | Populus alba |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 50 | about half-maximal activity at 25°C and 50°C, temperature profile | Populus alba |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
recombinant enzyme | Populus alba |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9.5 | activity range | Populus alba |