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Literature summary for 4.2.3.24 extracted from
- Cloning, E. coli expression and molecular analysis of amorpha-4,11-diene synthase from a high-yield strain of Artemisia annua L (2006), J. Integr. Plant Biol., 48, 1486-1492.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | amorpha-4,11-diene is a presursor of artemisinin, artemisinin and derivates are used for the treatment of malaria | Artemisia annua |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into the pET30a+ vector for expression in Escherichia coli | Artemisia annua |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | the apparent Km value is 3.4 mmol/l | Artemisia annua |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 69300 | - |
detemined by SDS-PAGE, recombinant protein extended with 17 amino acids after fusion to the His-tag | Artemisia annua |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-trans,6-trans-farnesyl diphosphate | Artemisia annua | - |
amorpha-4,11-diene + diphosphate | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Artemisia annua | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purified by immobilized metal affinity chromatography on a column loaded with Ni2+ | Artemisia annua |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| flower | - |
Artemisia annua | - |
| leaf | - |
Artemisia annua | - |
| stem | - |
Artemisia annua | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-trans,6-trans-farnesyl diphosphate | - |
Artemisia annua | amorpha-4,11-diene + diphosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ADS | - |
Artemisia annua |
| ADS1 | - |
Artemisia annua |
| amorpha-4,11-diene synthase | - |
Artemisia annua |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
activity assay | Artemisia annua |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
activity assay | Artemisia annua |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7 | - |
Artemisia annua |
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