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Literature summary for 4.2.3.22 extracted from
- Cloning and functional characterization of the germacradienol synthase (spterp13) from Streptomyces peucetius ATCC 27952 (2008), J. Microbiol. Biotechnol., 18, 1216-1220.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| full length recombinant protein is heterologously expressed as a his-tagged fusion protein in Escherichia coli | Streptomyces peucetius |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 10 mM are included in assay medium | Streptomyces peucetius |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 98000 | - |
recombinant Spterp13, consistent with the predicted mass of Spterp13 plus his-tag determined by SDS-PAGE analysis | Streptomyces peucetius |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2E,6E)-farnesyl diphosphate + H2O | Streptomyces peucetius | Mg-dependent conversion | (1E,4S,5E,7R)-germacra-1(10),5-dien-11-ol + diphosphate | - |
? | |
| additional information | Streptomyces peucetius | catalyze the Mg-dependent conversion of farnesyl diphosphate to the germacradienol, an essential step in the biosynthesis of geosmin | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces peucetius | B0FLN6 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purification by Co2+-affinity chromatography | Streptomyces peucetius |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | strain ATCC 27952 produces clinically important anthracycline chemotherapeutic agents of the polyketide class of antibiotics, daunorubicin and doxorubicin | Streptomyces peucetius | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2E,6E)-farnesyl diphosphate + H2O | Mg-dependent conversion | Streptomyces peucetius | (1E,4S,5E,7R)-germacra-1(10),5-dien-11-ol + diphosphate | - |
? | |
| additional information | catalyze the Mg-dependent conversion of farnesyl diphosphate to the germacradienol, an essential step in the biosynthesis of geosmin | Streptomyces peucetius | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| germacradienol synthase | - |
Streptomyces peucetius |
| GS | - |
Streptomyces peucetius |
| spterp13 | - |
Streptomyces peucetius |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Streptomyces peucetius |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.2 | - |
assay at | Streptomyces peucetius |
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