Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Lavandula angustifolia |
into the pGEM-T vector for sequencing and into the pGEX-T-1 vector for expression in Escherichia coli cells | Lavandula angustifolia |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0474 | - |
geranyl diphosphate | pH 7.0, 30°C | Lavandula angustifolia | |
0.0474 | - |
geranyl diphosphate | LaLIMS_RR, truncated protein | Lavandula angustifolia |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Lavandula angustifolia | |
Mg2+ | or Mn2+, reuired. Optimum concentraion 50 mM | Lavandula angustifolia | |
Mn2+ | the proportions of the products change significantly when Mn2+ is supplied instead of Mg2+ | Lavandula angustifolia | |
Mn2+ | or Mg2+, required. Optimum concentration 0.2 mM, 68% of the efficieny with Mg2+. Product pattern changes markedly to 46% limonene, 9% terpinolene, 23% alpha-pinene, 5% beta-myrcene and 4% alpha-phellandrene, when Mn2+ is supplied instead of Mg2+ | Lavandula angustifolia |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70300 | - |
x * 70300, SDS-PAGE | Lavandula angustifolia |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranyl diphosphate | Lavandula angustifolia | - |
(+)-(4R)-limonene + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lavandula angustifolia | Q2XSC6 | - |
- |
Purification (Comment) | Organism |
---|---|
using GST bind resin | Lavandula angustifolia |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | - |
Lavandula angustifolia | - |
leaf | - |
Lavandula angustifolia | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranyl diphosphate | - |
Lavandula angustifolia | (+)-(4R)-limonene + diphosphate | - |
? | |
geranyl diphosphate | - |
Lavandula angustifolia | (1R,5R)-(+)-alpha-pinene + diphosphate | - |
? | |
geranyl diphosphate | - |
Lavandula angustifolia | (1R,4S)-(+)-camphene + diphosphate | - |
? | |
geranyl diphosphate | - |
Lavandula angustifolia | alpha-phellandrene + diphosphate | - |
? | |
geranyl diphosphate | - |
Lavandula angustifolia | beta-myrcene + diphosphate | - |
? | |
geranyl diphosphate | - |
Lavandula angustifolia | terpinolene + diphosphate | - |
? | |
geranyl diphosphate | - |
Lavandula angustifolia | (R)-limonene + diphosphate | products are 39% (R)-limonene, 22% terpinolene, 16% (1R,5S)-camphene, 14% (1R,5R)-(+)-alpha-pinene, 8% betamyrcene and traces of alpha-phellandrene. Product pattern changes markedly to 46% limonene, 9% terpinolene, 23% alpha-pinene, 5% beta-myrcene and 4% alpha-phellandrene, when Mn2+ is supplied instead of Mg2+ | ? | |
additional information | no substrates: farnesyl diphosphate, geranylgeranyl diphosphate | Lavandula angustifolia | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 70300, SDS-PAGE | Lavandula angustifolia |
Synonyms | Comment | Organism |
---|---|---|
(R)-limonene synthase | - |
Lavandula angustifolia |
LaLIMS | - |
Lavandula angustifolia |
limonene synthase | - |
Lavandula angustifolia |
LIMS | - |
Lavandula angustifolia |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | - |
standard activity assay | Lavandula angustifolia |
30 | - |
- |
Lavandula angustifolia |
30 | - |
optimized assay, LaLIMS_RR, truncated protein | Lavandula angustifolia |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
geranyl diphosphate | LaLIMS_RR, truncated protein | Lavandula angustifolia |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Lavandula angustifolia |
7 | - |
optimized assay, LaLIMS_RR, truncated protein | Lavandula angustifolia |
7.5 | - |
standard activity assay | Lavandula angustifolia |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.255 | - |
geranyl diphosphate | pH 7.0, 30°C | Lavandula angustifolia |