Literature summary for 4.2.3.20 extracted from

Ohara, K.; Ujihara, T.; Endo, T.; Sato, F.; Yazaki, K.
Limonene production in tobacco with Perilla limonene synthase cDNA (2003), J. Exp. Bot., 54, 2635-2642.

Cloned(Commentary)

Cloned (Comment)	Organism
introduction of limonene synthase cDNA with three different sorting signals (to localize either in cytosol or the endoplasmic reticulum) in Nicotiana tabacum. Full-length and modified enzyme are subcloned in a binary vector under the El2 promoter, which is a strong constitutive promoter, to yield pBin-FullLC1, pBin-DEltaLC1 and pBin.ERLC1 to plastidial, cytosolic and endoplasmic localization, respectively. These plasmids are introduced via Agrobacterium tumefaciens into tobacco. More than 10 transgenic tobacco plants for each construct, i.e. 15 plastid localization, 17 cytosol localization, 11 endoplasmic reticulum localization are established	Perilla frutescens

Cloned (Comment)

Organism

introduction of limonene synthase cDNA with three different sorting signals (to localize either in cytosol or the endoplasmic reticulum) in Nicotiana tabacum. Full-length and modified enzyme are subcloned in a binary vector under the El2 promoter, which is a strong constitutive promoter, to yield pBin-FullLC1, pBin-DEltaLC1 and pBin.ERLC1 to plastidial, cytosolic and endoplasmic localization, respectively. These plasmids are introduced via Agrobacterium tumefaciens into tobacco. More than 10 transgenic tobacco plants for each construct, i.e. 15 plastid localization, 17 cytosol localization, 11 endoplasmic reticulum localization are established

Perilla frutescens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
plastid	-	Perilla frutescens	9536	-

Organism

Organism	UniProt	Comment	Textmining
Perilla frutescens	-	-	-

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Release 2023.1 (January 2023)