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Literature summary for 4.2.3.190 extracted from

  • Zerbe, P.; Chiang, A.; Dullat, H.; ONeil-Johnson, M.; Starks, C.; Hamberger, B.; Bohlmann, J.
    Diterpene synthases of the biosynthetic system of medicinally active diterpenoids in Marrubium vulgare (2014), Plant J., 79, 914-927 .
    View publication on PubMed
Search for more literature for 4.2.3.190

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Marrubium vulgare

Crystallization (Commentary)

Crystallization (Comment) Organism
ELS belongs to a subgroup of the diTPS TPS–e/f clade with unusual betaalpha-domain architecture Marrubium vulgare

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(13E)-8alpha-hydroxylabd-13-en-15-yl diphosphate Marrubium vulgare
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 manoyl oxide + diphosphate
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 ?
additional information Marrubium vulgare ELS is active in vitro and in vivo with three different prenyl diphosphate substrates forming the marrubiin precursor 9,13-epoxy-labd-14-ene, i.e. reaction of EC 4.2.3.189, manoyl oxide and miltiradiene, reaction of EC 4.2.3.131 ?
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 ?

Organism

Organism UniProt Comment Textmining
Marrubium vulgare A0A075FBG7
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-

Source Tissue

Source Tissue Comment Organism Textmining
flower transcript level is 4fold higher in leaf compared with flower Marrubium vulgare
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leaf transcript level is 4fold higher in leaf compared with flower Marrubium vulgare
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(13E)-8alpha-hydroxylabd-13-en-15-yl diphosphate
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 Marrubium vulgare manoyl oxide + diphosphate
-
 ?
additional information ELS is active in vitro and in vivo with three different prenyl diphosphate substrates forming the marrubiin precursor 9,13-epoxy-labd-14-ene, i.e. reaction of EC 4.2.3.189, manoyl oxide and miltiradiene, reaction of EC 4.2.3.131 Marrubium vulgare ?
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 ?

Synonyms

Synonyms Comment Organism
ELS
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 Marrubium vulgare

General Information

General Information Comment Organism
physiological function in vitro, in combination with the copalyl diphosphate-producing CPS3, ELS forms miltiradiene. When coupled with TPS2, ELS converts (13E)-8alpha-hydroxylabd-13-en-15-yl diphosphate into manoyl oxide. The coupled reaction of CPS1 and ELS leads to 80% (13epsilon)-9,13-epoxylabda-14-ene Marrubium vulgare