Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Fusarium verticillioides | |
cysteine | - |
Fusarium verticillioides | |
dithiothreitol | stimulates activity in presence of EDTA | Fusarium verticillioides |
General Stability | Organism |
---|---|
sensitive on freezing and thawing | Fusarium verticillioides |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-(N,N-dimethyl-N-octylammonium bromide)-p-methan-1-ol | Q-64 | Fusarium verticillioides | |
copalyl diphosphate | slight substrate inhibition at concentrations above 0.0007 mM | Fusarium verticillioides | |
Cu2+ | - |
Fusarium verticillioides | |
deoxycholate | - |
Fusarium verticillioides | |
EDTA | - |
Fusarium verticillioides | |
Hg2+ | - |
Fusarium verticillioides | |
iodacetamide | - |
Fusarium verticillioides | |
N,N-dimethylaminoethyl 2,2-diphenylphenylether | SKF 3301A | Fusarium verticillioides | |
N-ethylmaleimide | - |
Fusarium verticillioides | |
p-hydroxymercuribenzoate | - |
Fusarium verticillioides | |
Phosphone D | - |
Fusarium verticillioides | |
Phosphone S | - |
Fusarium verticillioides | |
sulfhydryl groups | - |
Fusarium verticillioides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
copalyl diphosphate | - |
Fusarium verticillioides |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | no effect | Fusarium verticillioides | |
Co2+ | can partially replace Mg2+ | Fusarium verticillioides | |
Mg2+ | required, optimum concentration 5 mM | Fusarium verticillioides | |
Mn2+ | can partially replace Mg2+ | Fusarium verticillioides | |
Ni2+ | can partially replace Mg2+ | Fusarium verticillioides |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
430000 | - |
sucrose density gradient centrifugation, crude enzyme | Fusarium verticillioides |
450000 | - |
sucrose density gradient centrifugation, purified enzyme | Fusarium verticillioides |
490000 | - |
gel filtration | Fusarium verticillioides |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ent-copalyl diphosphate | Fusarium verticillioides | involved in biosynthesis of gibberellins | ent-kaurene + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Fusarium verticillioides | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial, copurified with ent-kaurene synthase A (EC 5.5.1.13) activity | Fusarium verticillioides |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.123 | - |
purified enzyme | Fusarium verticillioides |
Storage Stability | Organism |
---|---|
-20°C, 25% glycerol, several months, no loss of activity for purified enzyme | Fusarium verticillioides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
copalyl diphosphate | - |
Fusarium verticillioides | sandaracopimaradiene + diphosphate | - |
? | |
ent-copalyl diphosphate | - |
Fusarium verticillioides | ent-kaurene + diphosphate | - |
? | |
ent-copalyl diphosphate | involved in biosynthesis of gibberellins | Fusarium verticillioides | ent-kaurene + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | part of bifunctional enzyme catalyzing the two step reaction from trans-geranylgeranyl-diphosphate to ent-kaurene, first step is catalyzed by EC 5.5.1.13 (ent-kaurene synthase A) | Fusarium verticillioides |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.9 | - |
TES or HEPES buffer | Fusarium verticillioides |
7.5 | - |
phosphate buffer | Fusarium verticillioides |