Cloned (Comment) | Organism |
---|---|
Site-directed mutagenesis is carried out via PCR amplification with overlapping mutagenic primers, and the mutant genes verify by complete sequencing. The resulting wild type and mutant genes are then transferred via directional recombination to the T7-promoter and N-terminal 6his fusion expression vector pDEST17. Use of the pDEST17 vector results in a 25 amino acid residue linker between the 6 His-tag and the cloned protein. | Abies grandis |
Protein Variants | Comment | Organism |
---|---|---|
D621A | eliminates class I activity | Abies grandis |
T769A | is active | Abies grandis |
T769G | is active | Abies grandis |
T769S | is active | Abies grandis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | 100 mM included in assay medium | Abies grandis | |
Mg2+ | terpene synthases (TPS) require divalent metal ion co-factors, typically magnesium | Abies grandis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
copalyl diphosphate | Abies grandis | class I terpene synthase reaction | abietadienes + diphosphate | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Abies grandis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purification using Ni-NTA His-bind resin in batch mode | Abies grandis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Abies grandis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
copalyl diphosphate | class I terpene synthase reaction | Abies grandis | abietadienes + diphosphate | - |
ir |
Synonyms | Comment | Organism |
---|---|---|
abietadiene synthase | - |
Abies grandis |
AGAS | - |
Abies grandis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Abies grandis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Abies grandis |