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Literature summary for 4.2.3.18 extracted from

  • Peters, R.J.; Croteau, R.B.
    Abietadiene synthase catalysis: mutational analysis of a prenyl diphosphate ionization-initiated cyclization and rearrangement (2002), Proc. Natl. Acad. Sci. USA, 99, 580-584.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.3.18

Protein Variants

Protein Variants Comment Organism
D621A
-
 Abies grandis
D625A no effect on geranylgeranyl diphosphate reaction, but lower turnover with copalyl diphosphate than wild-type Abies grandis
D766A no effect on geranylgeranyl diphosphate reaction, but lower turnover with copalyl diphosphate than wild-type Abies grandis
D845A no effect on geranylgeranyl diphosphate reaction, but lower turnover with copalyl diphosphate than wild-type Abies grandis
E589A no effect on geranylgeranyl diphosphate reaction, but lower turnover with copalyl diphosphate than wild-type Abies grandis
E699A no effect on geranylgeranyl diphosphate reaction, but lower turnover with copalyl diphosphate than wild-type Abies grandis
E773A no effect on geranylgeranyl diphosphate reaction, but lower turnover with copalyl diphosphate than wild-type Abies grandis
E778A no effect on geranylgeranyl diphosphate reaction, but lower turnover with copalyl diphosphate than wild-type Abies grandis
N765A no effect on geranylgeranyl diphosphate reaction, but lower turnover with copalyl diphosphate than wild-type Abies grandis
R584A no effect on geranylgeranyl diphosphate reaction, but lower turnover with copalyl diphosphate than wild-type Abies grandis
R586A no effect on geranylgeranyl diphosphate reaction, but lower turnover with copalyl diphosphate than wild-type Abies grandis
R762A no effect on geranylgeranyl diphosphate reaction, but lower turnover with copalyl diphosphate than wild-type Abies grandis
S721A no effect on geranylgeranyl diphosphate reaction, but lower turnover with copalyl diphosphate than wild-type Abies grandis
T617A no effect on geranylgeranyl diphosphate reaction, but lower turnover with copalyl diphosphate than wild-type Abies grandis
T769A no effect on geranylgeranyl diphosphate reaction, but lower turnover with copalyl diphosphate than wild-type Abies grandis
T848A no effect on geranylgeranyl diphosphate reaction, but lower turnover with copalyl diphosphate than wild-type Abies grandis
Y841F no effect on geranylgeranyl diphosphate reaction, but lower turnover with copalyl diphosphate than wild-type Abies grandis

Inhibitors

Inhibitors Comment Organism Structure
(13alpha)-N,13-dimethylpodocarp-8(14)-en-13-aminium chloride
-
 Abies grandis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0003
-
 copalyl diphosphate mutants E773A, E778A Abies grandis
0.0004
-
 copalyl diphosphate wild-type, mutant D766A Abies grandis
0.0005
-
 copalyl diphosphate mutant N765A Abies grandis
0.0007
-
 copalyl diphosphate mutants R762A, E699A, D621A Abies grandis
0.0008
-
 copalyl diphosphate mutants T848A, Y841F Abies grandis
0.001
-
 copalyl diphosphate mutants E589A, R584A Abies grandis
0.002
-
 copalyl diphosphate mutants R586A, T769A Abies grandis
0.003
-
 copalyl diphosphate mutant S721A Abies grandis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(+)-copalyl-diphosphate Abies grandis
-
 (-)-abietadiene + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Abies grandis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-copalyl-diphosphate
-
 Abies grandis (-)-abietadiene + diphosphate
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.05
-
 copalyl diphosphate mutant E589A Abies grandis
0.2
-
 copalyl diphosphate mutant E778A Abies grandis
0.4
-
 copalyl diphosphate mutant T617A Abies grandis