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Literature summary for 4.2.3.18 extracted from
- Abietadiene synthase catalysis: conserved residues involved in protonation-initiated cyclization of geranylgeranyl diphosphate to (+)-copalyl diphosphate (2002), Biochemistry, 41, 1836-1842.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D361A | lower turnover with geranylgeranyl diphosphate than wild-type | Abies grandis |
| D402A | lower turnover with geranylgeranyl diphosphate than wild-type | Abies grandis |
| D402E | lower turnover with geranylgeranyl diphosphate than wild-type | Abies grandis |
| D402N | lower turnover with geranylgeranyl diphosphate than wild-type | Abies grandis |
| D404A | - |
Abies grandis |
| D404E | lower turnover with geranylgeranyl diphosphate than wild-type | Abies grandis |
| D404N | lower turnover with geranylgeranyl diphosphate than wild-type | Abies grandis |
| D405A | lower turnover with geranylgeranyl diphosphate than wild-type | Abies grandis |
| D405E | lower turnover with geranylgeranyl diphosphate than wild-type | Abies grandis |
| D405N | lower turnover with geranylgeranyl diphosphate than wild-type | Abies grandis |
| E499A | lower turnover with geranylgeranyl diphosphate than wild-type | Abies grandis |
| R365A | lower turnover with geranylgeranyl diphosphate than wild-type | Abies grandis |
| R411A | lower turnover with geranylgeranyl diphosphate than wild-type | Abies grandis |
| R454A | lower turnover with geranylgeranyl diphosphate than wild-type | Abies grandis |
| W358A | lower turnover with geranylgeranyl diphosphate than wild-type | Abies grandis |
| Y520A | lower turnover with geranylgeranyl diphosphate than wild-type | Abies grandis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 14,15-dihydro-15-aza-geranylgeranyl diphosphate | - |
Abies grandis |  |
| geranylgeranyl diphosphate | - |
Abies grandis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0002 | - |
copalyl diphosphate | mutants D404N, R411A | Abies grandis | |
| 0.0003 | - |
copalyl diphosphate | mutants D402A, D402N, D404E | Abies grandis | |
| 0.0004 | - |
copalyl diphosphate | mutants W358A, D361A, D404A, D405E | Abies grandis | |
| 0.0005 | - |
copalyl diphosphate | mutants D402E, D405E, R454A, E499A Y520A | Abies grandis | |
| 0.0007 | - |
copalyl diphosphate | mutants D405N, R365A | Abies grandis | |
| 132 | - |
copalyl diphosphate | - |
Abies grandis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 10 mM selectively enables the conversion of copalyl diphosphate | Abies grandis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (+)-copalyl-diphosphate | Abies grandis | - |
(-)-abietadiene + diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Abies grandis | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (+)-copalyl-diphosphate | - |
Abies grandis | (-)-abietadiene + diphosphate | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2 | - |
copalyl diphosphate | mutants D361A, D402A, D402E, D404N, D404A, D404E, D404N, D405A, D405E, D405N, D621A, E499A, R365A, R411A, R454A, W358A, Y520A | Abies grandis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.7 | - |
with copalyl diphosphate as substrate | Abies grandis |
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Release 2023.1 (January 2023)
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