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Literature summary for 4.2.3.155 extracted from
- Evolutionary divergence of sedoheptulose 7-phosphate cyclases leads to several distinct cyclic products (2012), J. Am. Chem. Soc., 134, 12219-12229.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Actinosynnema mirum |
| expression in Escherichia coli | Stigmatella aurantiaca |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0386 | - |
D-sedoheptulose 7-phosphate | pH 7.0, 28°C | Stigmatella aurantiaca |  |
| 0.0599 | - |
D-sedoheptulose 7-phosphate | pH 7.0, 28°C | Actinosynnema mirum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | the enzyme requires a divalent metal ion (Zn2+ or Co2+) | Actinosynnema mirum | |
| Co2+ | the enzyme requires a divalent metal ion (Zn2+ or Co2+) | Stigmatella aurantiaca | |
| Zn2+ | the enzyme requires a divalent metal ion (Zn2+ or Co2+) | Actinosynnema mirum | |
| Zn2+ | the enzyme requires a divalent metal ion (Zn2+ or Co2+) | Stigmatella aurantiaca | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 41009 | - |
x * 41009, calculated from sequence | Actinosynnema mirum |
| 43439 | - |
x * 43439, calculated from sequence | Stigmatella aurantiaca |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-sedoheptulose 7-phosphate | Actinosynnema mirum | the enzyme produces 2-epi-valiolone, which is believed to function as a precursor in aminocyclitol biosynthesis | 2-epi-valiolone + phosphate | - |
? | |
| D-sedoheptulose 7-phosphate | Stigmatella aurantiaca | the enzyme produces 2-epi-valiolone, which is believed to function as a precursor in aminocyclitol biosynthesis | 2-epi-valiolone + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Actinosynnema mirum | C6WFL3 | - |
- |
| Stigmatella aurantiaca | Q08VU0 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Actinosynnema mirum |
- |
Stigmatella aurantiaca |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-sedoheptulose 7-phosphate | - |
Actinosynnema mirum | 2-epi-valiolone + phosphate | 2-epi-valiolone i.e. (2S,3S,4S,5S)-2,3,4,5-tetrahydroxy-5-(hydroxymethyl)cyclohexan-1-one | ? | |
| D-sedoheptulose 7-phosphate | - |
Stigmatella aurantiaca | 2-epi-valiolone + phosphate | 2-epi-valiolone i.e. (2S,3S,4S,5S)-2,3,4,5-tetrahydroxy-5-(hydroxymethyl)cyclohexan-1-one | ? | |
| D-sedoheptulose 7-phosphate | the enzyme produces 2-epi-valiolone, which is believed to function as a precursor in aminocyclitol biosynthesis | Actinosynnema mirum | 2-epi-valiolone + phosphate | - |
? | |
| D-sedoheptulose 7-phosphate | the enzyme produces 2-epi-valiolone, which is believed to function as a precursor in aminocyclitol biosynthesis | Stigmatella aurantiaca | 2-epi-valiolone + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 41009, calculated from sequence | Actinosynnema mirum |
| ? | x * 43439, calculated from sequence | Stigmatella aurantiaca |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| aroB | - |
Actinosynnema mirum |
| aroB | - |
Stigmatella aurantiaca |
| EVS | - |
Actinosynnema mirum |
| EVS | - |
Stigmatella aurantiaca |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 28 | - |
assay at | Actinosynnema mirum |
| 28 | - |
assay at | Stigmatella aurantiaca |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.033 | - |
D-sedoheptulose 7-phosphate | pH 7.0, 28°C | Actinosynnema mirum | |
| 0.062 | - |
D-sedoheptulose 7-phosphate | pH 7.0, 28°C | Stigmatella aurantiaca | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Actinosynnema mirum |
| 7 | - |
assay at | Stigmatella aurantiaca |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | the enzyme requires an NAD+ cofactor, which is transiently reduced during the reaction | Actinosynnema mirum | |
| NAD+ | the enzyme requires an NAD+ cofactor, which is transiently reduced during the reaction | Stigmatella aurantiaca | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the enzyme produces 2-epi-valiolone, which is believed to function as a precursor in aminocyclitol biosynthesis | Actinosynnema mirum |
| metabolism | the enzyme produces 2-epi-valiolone, which is believed to function as a precursor in aminocyclitol biosynthesis | Stigmatella aurantiaca |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.556 | - |
D-sedoheptulose 7-phosphate | pH 7.0, 28°C | Actinosynnema mirum | |
| 1.6 | - |
D-sedoheptulose 7-phosphate | pH 7.0, 28°C | Stigmatella aurantiaca | |
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Release 2023.1 (January 2023)
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