Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Methanocaldococcus jannaschii |
Crystallization (Comment) | Organism |
---|---|
molecular docking of glycerinaldehyde 3-phosphate into the active site | Methanocaldococcus jannaschii |
Protein Variants | Comment | Organism |
---|---|---|
D151N | mutation of strictly conserved residue around the active site, complete inactivation | Methanocaldococcus jannaschii |
D25N | mutation of strictly conserved residue around the active site, complete inactivation | Methanocaldococcus jannaschii |
K155R | kcat/KM value comparable to the wild-type | Methanocaldococcus jannaschii |
K27R | mutation of strictly conserved residue around the active site, complete inactivation | Methanocaldococcus jannaschii |
K85R | mutation of strictly conserved residue around the active site, complete inactivation | Methanocaldococcus jannaschii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.035 | - |
D-glyceraldehyde 3-phosphate | wild-type, pH 7.0, 70°C | Methanocaldococcus jannaschii | |
0.048 | - |
D-glyceraldehyde 3-phosphate | mutant K155R, pH 7.0, 70°C | Methanocaldococcus jannaschii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
x * 25000, SDS-PAGE | Methanocaldococcus jannaschii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | Q58499 | - |
- |
Methanocaldococcus jannaschii DSM 2661 | Q58499 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2 D-glyceraldehyde 3-phosphate = (5-formylfuran-3-yl)methyl phosphate + phosphate + 2 H2O | a phosphate elimination reaction and a triose phosphate isomerase-like reaction occur at the glyceraldehyde 3-phosphate binding site I and II, respectively, prior to the aldol condensation between the enzyme-bound enol form of methylglyoxal and dihydroxyacetone phosphate, after which the catalytic cycle is completed by a cyclization and two dehydration reactions assisted by several general acids/bases at the same active site | Methanocaldococcus jannaschii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 D-glyceraldehyde 3-phosphate | - |
Methanocaldococcus jannaschii | (5-formylfuran-3-yl)methyl phosphate + phosphate + 2 H2O | - |
? | |
2 D-glyceraldehyde 3-phosphate | - |
Methanocaldococcus jannaschii DSM 2661 | (5-formylfuran-3-yl)methyl phosphate + phosphate + 2 H2O | - |
? | |
D-glyceraldehyde 3-phosphate | - |
Methanocaldococcus jannaschii | (5-formylfuran-3-yl)methyl phosphate + phosphate + H2O | - |
? | |
D-glyceraldehyde 3-phosphate | - |
Methanocaldococcus jannaschii DSM 2661 | (5-formylfuran-3-yl)methyl phosphate + phosphate + H2O | - |
? | |
additional information | no substrates: pyruvate, methylglyoxal, phosphoenolpyruvate, oxaloacetic acid, dihydroxyacetone, and dihydroxyacetone phosphate | Methanocaldococcus jannaschii | ? | - |
? | |
additional information | no substrates: pyruvate, methylglyoxal, phosphoenolpyruvate, oxaloacetic acid, dihydroxyacetone, and dihydroxyacetone phosphate | Methanocaldococcus jannaschii DSM 2661 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 25000, SDS-PAGE | Methanocaldococcus jannaschii |
Synonyms | Comment | Organism |
---|---|---|
4-(hydroxymethyl)-2-furancarboxaldehyde-phosphate synthase | - |
Methanocaldococcus jannaschii |
4-HFC-P synthase | - |
Methanocaldococcus jannaschii |
mfnB | - |
Methanocaldococcus jannaschii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.022 | - |
D-glyceraldehyde 3-phosphate | mutant K155R, pH 7.0, 70°C | Methanocaldococcus jannaschii | |
0.026 | - |
D-glyceraldehyde 3-phosphate | wild-type, pH 7.0, 70°C | Methanocaldococcus jannaschii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.46 | - |
D-glyceraldehyde | mutant K155R, pH 7.0, 70°C | Methanocaldococcus jannaschii | |
0.74 | - |
D-glyceraldehyde | wild-type, pH 7.0, 70°C | Methanocaldococcus jannaschii |