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Literature summary for 4.2.3.152 extracted from

  • Choi, W.S.; Wu, X.; Choeng, Y.H.; Mahmud, T.; Jeong, B.C.; Lee, S.H.; Chang, Y.K.; Kim, C.J.; Hong, S.K.
    Genetic organization of the putative salbostatin biosynthetic gene cluster including the 2-epi-5-epi-valiolone synthase gene in Streptomyces albus ATCC 21838 (2008), Appl. Microbiol. Biotechnol., 80, 637-645.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Streptomyces albus
overexpressed in Escherichia coli Streptomyces albus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50500
-
x * 50500, SDS-PAGE, His-tagged recombinant protein Streptomyces albus
50550
-
x * 50550, polyhistidine-tagged protein, SDS-PAGE Streptomyces albus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-sedoheptulose 7-phosphate Streptomyces albus the enzyme is involved in biosynthesis of 2-epi-5-epi-valiolone a precursor in the biosynthesis of C7N-aminocyclitol-containing natural products, such as validamycin and acarbose 2-epi-5-epi-valiolone + phosphate
-
?
D-sedoheptulose 7-phosphate Streptomyces albus ATCC 21838 the enzyme is involved in biosynthesis of 2-epi-5-epi-valiolone a precursor in the biosynthesis of C7N-aminocyclitol-containing natural products, such as validamycin and acarbose 2-epi-5-epi-valiolone + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces albus A8D7K2
-
-
Streptomyces albus ATCC 21838 A8D7K2
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptomyces albus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-sedoheptulose 7-phosphate the enzyme is involved in biosynthesis of 2-epi-5-epi-valiolone a precursor in the biosynthesis of C7N-aminocyclitol-containing natural products, such as validamycin and acarbose Streptomyces albus 2-epi-5-epi-valiolone + phosphate
-
?
D-sedoheptulose 7-phosphate SalQ has high specificity for sedoheptulose 7-phosphate as substrate. No activity with D-glucose 6-phosphate, D-fructose 6-phosphate, D-mannose 6-phosphate, 3-deoxy-D-arabino-heptulosonate 7-phosphate and D-ribose 5-phosphate Streptomyces albus 2-epi-5-epi-valiolone + phosphate
-
?
D-sedoheptulose 7-phosphate the enzyme is involved in biosynthesis of 2-epi-5-epi-valiolone a precursor in the biosynthesis of C7N-aminocyclitol-containing natural products, such as validamycin and acarbose Streptomyces albus ATCC 21838 2-epi-5-epi-valiolone + phosphate
-
?
D-sedoheptulose 7-phosphate SalQ has high specificity for sedoheptulose 7-phosphate as substrate. No activity with D-glucose 6-phosphate, D-fructose 6-phosphate, D-mannose 6-phosphate, 3-deoxy-D-arabino-heptulosonate 7-phosphate and D-ribose 5-phosphate Streptomyces albus ATCC 21838 2-epi-5-epi-valiolone + phosphate
-
?
additional information no substrates: amino 3-deoxy-D-arabino-heptulosonate 7-phosphate, 3-deoxy-D-arabino-heptulosonate 7-phosphate, glucose 6-phosphate, fructose 6-phosphate, mannose 6-phosphate, and ribose 5-phosphate Streptomyces albus ?
-
?
additional information no substrates: amino 3-deoxy-D-arabino-heptulosonate 7-phosphate, 3-deoxy-D-arabino-heptulosonate 7-phosphate, glucose 6-phosphate, fructose 6-phosphate, mannose 6-phosphate, and ribose 5-phosphate Streptomyces albus ATCC 21838 ?
-
?
sedoheptulose 7-phosphate
-
Streptomyces albus 2-epi-5-epi-valiolone + phosphate
-
?
sedoheptulose 7-phosphate
-
Streptomyces albus ATCC 21838 2-epi-5-epi-valiolone + phosphate
-
?

Subunits

Subunits Comment Organism
? x * 50550, polyhistidine-tagged protein, SDS-PAGE Streptomyces albus
? x * 50500, SDS-PAGE, His-tagged recombinant protein Streptomyces albus

Synonyms

Synonyms Comment Organism
SalQ
-
Streptomyces albus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Streptomyces albus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Streptomyces albus

General Information

General Information Comment Organism
physiological function the enzyme is involved in biosynthesis of 2-epi-5-epi-valiolone a precursor in the biosynthesis of C7N-aminocyclitol-containing natural products, such as validamycin and acarbose Streptomyces albus