Cloned (Comment) | Organism |
---|---|
gene TPS2, cloning from a cDNA library, sequence comparisons and phylogenetic analysis and tree, quantitative RT-PCR expression analysis, recombinant expression of His-tagged enzyme without after removing the N-terminal plastid targeting sequence in Escherichia coli strain BL21(DE3) | Artemisia annua |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the N-terminal signal peptide of AaTPS2 (46 amino acid residues) before RR motif is truncated and recombinantly expressed in Escherichia coli strain BL21(DE3) | Artemisia annua |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Artemisia annua | |
0.00825 | - |
geranyl diphosphate | pH 7.0, 30°C, recombinant enzyme | Artemisia annua |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, Mg2+ is the highly preferred divalent cation | Artemisia annua | |
Mn2+ | low activity | Artemisia annua | |
additional information | although both Mg2+ and Mn2+ are able to support the catalytic activity of enzyme AaTPS2, an altered product spectrum is observed in the presence of Mn2+ for AaTPS2 | Artemisia annua |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranyl diphosphate | Artemisia annua | - |
beta-myrcene + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Artemisia annua | A0A068L9J0 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme without N-terminal signal peptide from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Artemisia annua |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Artemisia annua | - |
additional information | quantitative RT-PCR expression analysis in plants tissues. AaTPS2 exhibits the highest expression level in stem, and low expression in the other tissues | Artemisia annua | - |
root | - |
Artemisia annua | - |
stem | highest expression level of AaTPS2 | Artemisia annua | - |
trichome | glandular | Artemisia annua | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranyl diphosphate | - |
Artemisia annua | beta-myrcene + diphosphate | - |
? | |
additional information | GC-MS analysis and identification of products from enzyme catalysis. Enzyme AaTPS2 produces a single product, beta-myrcene. No activity with farnesyl diphosphate or geranylgeranyl diphosphate | Artemisia annua | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AaTPS2 | - |
Artemisia annua |
TPS2 | - |
Artemisia annua |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Artemisia annua |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.52 | - |
geranyl diphosphate | pH 7.0, 30°C, recombinant enzyme | Artemisia annua |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Artemisia annua |
Organism | Comment | Expression |
---|---|---|
Artemisia annua | AaTPS2 is upregulated after mechanical wounding, but treatments with phytohormones (salicylic acid, methyl jasmonate, and gibberellin) does not affect the expression of AaTPS2 | up |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme AaTPS2 belongs to the TPS superfamily, subclade TPS-b | Artemisia annua |
metabolism | in Artemisia annua, three monoterpene synthases AaTPS2, AaTPS5, and AaTPS6, produce beta-myrcene, camphene, and 1,8-cineole as the major products, respectively, analysis of monoterpenes and sesquiterpenes produced by Artemisia annua plants, overview | Artemisia annua |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
63.03 | - |
geranyl diphosphate | pH 7.0, 30°C, recombinant enzyme | Artemisia annua |