Cloned (Comment) | Organism |
---|---|
recombinantly expressed in Escherichia coli | Abies balsamea |
Crystallization (Comment) | Organism |
---|---|
homology modeling based on PDB entry 3p5pA. The active site residues Asp-48, Leu617, Phe696, and Gly723 are potentially important for the specificity | Abies balsamea |
Protein Variants | Comment | Organism |
---|---|---|
D402A/D404A | mutation results in complete loss of activity | Abies balsamea |
D402A/D404A | monofunctional class II protein variant, complete loss of enzymatic activity with geranylgeranyl diphosphate as substrate | Abies balsamea |
D621A | monofunctional class I protein variant, mutant produces trace amounts of copalyl diphosphate plus labda-13-en-8,15-diol | Abies balsamea |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Abies balsamea | 9507 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Abies balsamea | H8ZM73 | - |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Abies balsamea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(13E)-8alpha-hydroxylabd-13-en-15-yl diphosphate | formation of cis-abienol proceeds via cyclization and hydroxylation at carbon C-8 of a postulated carbocation intermediate in the class II active site, followed by cleavage of the diphosphate group and termination of the reaction sequence without further cyclization in the class I active site | Abies balsamea | cis-abienol + diphosphate | - |
? | |
additional information | using (E,E,E)-geranylgeranyl diphosphate as a substrate AbdiTPS4 shows a unique single-peak product profile, which corresponds to cis-abienol 4 | Abies balsamea | ? | - |
? | |
additional information | bifunctional class I/II enzyme | Abies balsamea | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AbCAS | - |
Abies balsamea |
AbdiTPS4 | - |
Abies balsamea |
CAS | - |
Abies balsamea |
cis-abienol synthase | - |
Abies balsamea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Abies balsamea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Abies balsamea |