Cloned (Comment) | Organism |
---|---|
DELTA84 pseudo-mature form of abietadiene synthase is expressed in Escherichia coli C41 and B834(DE3) cells | Abies grandis |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 24% (w/v) PEG 8000, 0.1 M sodium citrate, pH 5.1, 0.1 M dibasic ammonium phosphate | Abies grandis |
Protein Variants | Comment | Organism |
---|---|---|
D621A | the mutation selectively abrogates abietadiene synthase class I activity | Abies grandis |
N451A | the mutant shows reduced class I abietadiene synthase activity compared to the wild type enzyme | Abies grandis |
R356A | the mutant shows reduced class I abietadiene synthase activity compared to the wild type enzyme | Abies grandis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0007 | - |
(+)-copalyl diphosphate | mutant enzyme R356A, pH and temperature not specified in the publication | Abies grandis | |
0.0014 | - |
(+)-copalyl diphosphate | mutant enzyme N451A, pH and temperature not specified in the publication | Abies grandis | |
0.002 | - |
(+)-copalyl diphosphate | wild type enzyme, pH and temperature not specified in the publication | Abies grandis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Abies grandis | Q38710 | - |
- |
Purification (Comment) | Organism |
---|---|
type II ceramic hydroxyapatite column chromatography and Mono Q column chromatography | Abies grandis |
Storage Stability | Organism |
---|---|
-80°C, 10 mM Bis-Tris, pH 6.8, 10% (v/v) glycerol, 150 mM KCl, 10 mM MgCl2, and 5 mM dithiothreitol, several months, without significant loss of activity | Abies grandis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-copalyl diphosphate | - |
Abies grandis | neoabietadiene + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | x-ray crystallography | Abies grandis |
Synonyms | Comment | Organism |
---|---|---|
abietadiene synthase | cf. EC 4.2.3.18 | Abies grandis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.1 | - |
(+)-copalyl diphosphate | mutant enzyme R356A, pH and temperature not specified in the publication | Abies grandis | |
1.9 | - |
(+)-copalyl diphosphate | mutant enzyme N451A, pH and temperature not specified in the publication | Abies grandis | |
2.6 | - |
(+)-copalyl diphosphate | wild type enzyme, pH and temperature not specified in the publication | Abies grandis |