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Literature summary for 4.2.3.13 extracted from

  • Chen, X.Y.; Chen, Y.; Heinstein, P.; Davisson, V.J.
    Cloning, expression, and characterization of (+)-delta-cadinene synthase: a catalyst for cotton phytoalexin biosynthesis (1995), Arch. Biochem. Biophys., 324, 255-266.
    View publication on PubMed
Search for more literature for 4.2.3.13

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli of a cDNA isolated and amplified from a cell culture infected with Verticillium dahliae Gossypium arboreum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0017
-
 2-trans,6-trans-farnesyl diphosphate pH 7, 30°C, fusion protein composed of the pXC1-encoded protein and the histidine leader peptide derived from pET28 Gossypium arboreum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Gossypium arboreum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64100
-
 calculated from DNA sequence Gossypium arboreum
64120
-
 calculated from DNA sequence Gossypium arboreum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-trans,6-trans-farnesyl diphosphate Gossypium arboreum the fungal-elicited production of a (+)-delta-cadinene synthase is consistent with a role for this enzyme as the first committed step in the pathways leading to the related phytoalexins gossypol and lacinilene C in cotton (+)-delta-cadinene + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Gossypium arboreum Q39760 cv. Nanking
-
Gossypium arboreum Q39761 cv. Nanking
-

Purification (Commentary)

Purification (Comment) Organism
purification of a recombinant enzyme using histidine affinity chromatography Gossypium arboreum

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture time-dependent 10fold increase in mRNA in response to a challenge by preparation from Verticillium dahliae Gossypium arboreum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.03
-
 recombinant protein Gossypium arboreum

Storage Stability

Storage Stability Organism
-20°C, stable for at least 4 weeks Gossypium arboreum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-trans,6-trans-farnesyl diphosphate
-
 Gossypium arboreum (+)-delta-cadinene + diphosphate
-
 ?
2-trans,6-trans-farnesyl diphosphate the fungal-elicited production of a (+)-delta-cadinene synthase is consistent with a role for this enzyme as the first committed step in the pathways leading to the related phytoalexins gossypol and lacinilene C in cotton Gossypium arboreum (+)-delta-cadinene + diphosphate
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.033
-
 2-trans,6-trans-farnesyl diphosphate fusion protein composed of the pXC1-encoded protein and the histidine leader peptide derived from pET28, pH 7, 30°C Gossypium arboreum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.7 8
-
 Gossypium arboreum