Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | increasing the reaction temperature to 37°C decreases the fidelity of Cop4. At this temperature Cop4 generates a relative larger fraction of products beta-cubebene, sativene, delta-cadinene and beta-copaene, that are derived from a cadinyl cation intermediate | Coprinopsis cinerea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic analysis, overview | Coprinopsis cinerea |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, substitution of Mg2+ with Mn2+ as the divalent metal ion shifts the product profile of Cop4 to germacrene D, disfavoring subsequent ring closures that produce the cadinyl cation and its tricyclic descendents. Two consensus sequences - an aspartate rich DDXXD/E and a NSE/DTE motif - located at the entrance of the active site coordinate a trinuclear Mg2+ cluster that ligands the diphosphate moiety of the isoprenoid substrate, positions the isoprenyl chain in the binding pocket and triggers closure of the active site along with diphosphate cleavage to generate an initial transoid, allylic carbocation | Coprinopsis cinerea | |
Mn2+ | substitution of Mg2+ with Mn2+ as the divalent metal ion shifts the product profile of Cop4 to germacrene D, disfavoring subsequent ring closures that produce the cadinyl cation and its tricyclic descendents | Coprinopsis cinerea | |
NaCl | does not affect the product specificity of Cop4 significantly at 1 M | Coprinopsis cinerea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate | Coprinopsis cinerea | - |
(+)-sativene + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Coprinopsis cinerea | A8NU13 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate | - |
Coprinopsis cinerea | (+)-sativene + diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate | conversion of (E,E)-farnesyl diphosphate proceeds via an (E,E)-germacradienyl carbocation in the case of Cop4 | Coprinopsis cinerea | (+)-sativene + diphosphate | - |
? | |
(2Z,6E)-farnesyl diphosphate | conversion of (E,E)-farnesyl diphosphate proceeds via a (6S)-beta-bisabolene carbocation in the case of Cop4 | Coprinopsis cinerea | (+)-sativene + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | structural modeling, structure-function relationship, overview. Cop4 has a large binding pocket in the open conformation | Coprinopsis cinerea |
Synonyms | Comment | Organism |
---|---|---|
Cop4 | - |
Coprinopsis cinerea |
sesquiterpene synthase | - |
Coprinopsis cinerea |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
changing the pH of the reaction drastically alters the fidelity of Cop4 and makes it a highly selective enzyme. Increasing the reaction temperature to 37°C decreases the fidelity of Cop4. At this temperature Cop4 generates a relative larger fraction of products beta-cubebene, sativene, delta-cadinene and beta-copaene, that are derived from a cadinyl cation intermediate. The histidine side chain in the Cop4 loop, in particular, has a strong impact on the net charge of the loop at different pH values | Coprinopsis cinerea |
General Information | Comment | Organism |
---|---|---|
additional information | structural modeling, structure-function relationship, overview. Changing the pH of the reaction drastically alters the fidelity of Cop4 and makes it a highly selective enzyme | Coprinopsis cinerea |