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Literature summary for 4.2.3.124 extracted from

  • Kogure, T.; Wakisaka, N.; Takaku, H.; Takagi, M.
    Efficient production of 2-deoxy-scyllo-inosose from D-glucose by metabolically engineered recombinant Escherichia coli (2007), J. Biotechnol., 129, 502-509.
    View publication on PubMed
Search for more literature for 4.2.3.124

Cloned(Commentary)

Cloned (Comment) Organism
gene DOIS, expression in Escherichia coli strain GI724 from plasmid pGA-btrC Niallia circulans

Protein Variants

Protein Variants Comment Organism
additional information introduction of DOIS gene btrC from Bacillus circulans and disruption of genes for phosphoglucose isomerase, D-glucose 6-phosphate dehydrogenase, and phosphoglucomutase, pgi, zwf and pgm, respectively, in Escherichia coli strain GI724. While GI724DELTAP/pGA-btrC and GI724DELTAPP/pGA-btrC possess functional Zwf and thus can catabolize glucose via the pentose phosphate pathway, glucose catabolism is completely inhibited in GI724DELTAPZ/pGA-btrC and GI724DELTAPZP/pGA-btrC due to the deletion of both pgi and zwf genes Niallia circulans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glucose 6-phosphate Niallia circulans
-
 2-deoxy-L-scyllo-inosose + phosphate
-
 ?
D-glucose 6-phosphate Niallia circulans SANK72073
-
 2-deoxy-L-scyllo-inosose + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Niallia circulans Q9S5E2 gene btrC
-
Niallia circulans SANK72073 Q9S5E2 gene btrC
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose 6-phosphate
-
 Niallia circulans 2-deoxy-L-scyllo-inosose + phosphate
-
 ?
D-glucose 6-phosphate
-
 Niallia circulans SANK72073 2-deoxy-L-scyllo-inosose + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
DOIS
-
 Niallia circulans

General Information

General Information Comment Organism
metabolism 2-deoxy-scyllo-inosose synthase, DOIS, is a key enzyme in the biosynthesis of 2-deoxystreptamine-containing aminocyclitol antibiotics Niallia circulans