Literature summary for 4.2.3.124 extracted from

Tamegai, H.; Sawada, H.; Nango, E.; Aoki, R.; Hirakawa, H.; Iino, T.; Eguchi, T.
Roles of a 20 kDa protein associated with a carbocycle-forming enzyme involved in aminoglycoside biosynthesis in primary and secondary metabolism (2010), Biosci. Biotechnol. Biochem., 74, 1215-1219.

Search for more literature for 4.2.3.124

Application

Application	Comment	Organism
industry	the enzyme converts the sustainable source glucose 6-phosphate to the carbocycle 2-deoxy-scylla-inosose, which easily aromatizes to yield catechol	Niallia circulans

Cloned(Commentary)

Cloned (Comment)	Organism
complementation of a btrC2 disruption mutant by expression of wild-type btrC2 from plasmid pHB201 in Bacillus circulans	Niallia circulans

Protein Variants

Protein Variants	Comment	Organism
additional information	complementation of a btrC2 disruption mutant by expression of wild-type btrC2 from plasmid pHB201 in Bacillus circulans	Niallia circulans

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	required	Niallia circulans

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
20000	-	1 * 40000, catalytic BtrC, + 1 * 20000, noncatalytic BtrC2, SDS-PAGE	Niallia circulans
40000	-	1 * 40000, catalytic BtrC, + 1 * 20000, noncatalytic BtrC2, SDS-PAGE	Niallia circulans

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-glucose 6-phosphate	Niallia circulans	-	2-deoxy-L-scyllo-inosose + phosphate	-	?
D-glucose 6-phosphate	Niallia circulans SANK72073	-	2-deoxy-L-scyllo-inosose + phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Niallia circulans	Q9S5E2	genes btrC and btrC2	-
Niallia circulans SANK72073	Q9S5E2	genes btrC and btrC2	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-glucose 6-phosphate	-	Niallia circulans	2-deoxy-L-scyllo-inosose + phosphate	-	?
D-glucose 6-phosphate	-	Niallia circulans SANK72073	2-deoxy-L-scyllo-inosose + phosphate	-	?

Subunits

Subunits	Comment	Organism
heterodimer	1 * 40000, catalytic BtrC, + 1 * 20000, noncatalytic BtrC2, SDS-PAGE	Niallia circulans

Synonyms

Synonyms	Comment	Organism
DOI synthase	-	Niallia circulans

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	required	Niallia circulans

Expression

Organism	Comment	Expression
Niallia circulans	BtrC expression is highly reduced by supplementation with 0.01 mg/ml pyridoxal	down

General Information

General Information	Comment	Organism
physiological function	the role of the 20 kDa non-catalytic protein BtrC2, associated with the DOI synthase, in butirosin biosynthesis is to stabilize the synthase by complexing as heterodimer with BtrC allowing the stable production of butirosin for long periods. BtrC2 is also involved in the biosynthesis of vitamin B6 in primary metabolism	Niallia circulans

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)