Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-glucose 6-homophosphonate | competitive inhibition versus D-glucose 6-phosphate | Niallia circulans | |
D-glucose 6-phosphonate | competitive inhibition versus D-glucose 6-phosphate, hydride transfer from 6-phosphonate to NAD+ | Niallia circulans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.21 | - |
D-glucose 6-phosphate | pH 7.7, 46°C | Niallia circulans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | required | Niallia circulans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate | Niallia circulans | the enzyme converts ubiquitous D-glucose 6-phosphate into the specific carbocycle, 2-deoxy-scyllo-inosose with an aid of NAD+-NADH recycling | 2-deoxy-L-scyllo-inosose + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Niallia circulans | Q9S5E2 | gene btrC | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
D-glucose 6-phosphate = 2-deoxy-L-scyllo-inosose + phosphate | DOIS catalyzes the multi-step and direct cyclization reaction of G-6-P consisting five crucial transformations, which include the initial NAD+-dependent dehydrogenation at C-4, the elimination of a phosphate from C-5 and C-6, the hydride comeback to C-4 to form a cyclic enol ether, and the finial aldol-type intramolecular condensation between C-6 and C-1 | Niallia circulans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-deoxy-D-glucose 6-phosphate | low activity | Niallia circulans | 2,5-dideoxy-L-scyllo-inosose + phosphate | - |
? | |
3-deoxy-D-glucose 6-phosphate | low activity | Niallia circulans | 2,4-dideoxy-L-scyllo-inosose + phosphate | - |
? | |
D-glucose 6-phosphate | the enzyme converts ubiquitous D-glucose 6-phosphate into the specific carbocycle, 2-deoxy-scyllo-inosose with an aid of NAD+-NADH recycling | Niallia circulans | 2-deoxy-L-scyllo-inosose + phosphate | - |
? | |
D-glucose 6-phosphate | pro-R stereospecific hydride transfer by NAD+-catalyzed 2-deoxy-scyllo-inosose synthase | Niallia circulans | 2-deoxy-L-scyllo-inosose + phosphate | - |
? | |
additional information | specific hydrogen-bond accepting and donating interactions between the hydroxy groups at C-2 and C-3 of the substrate and the enzyme are responsible for the recognition | Niallia circulans | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
btrC | - |
Niallia circulans |
DOIS | - |
Niallia circulans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
46 | - |
assay at | Niallia circulans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.7 | - |
assay at | Niallia circulans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | required | Niallia circulans |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
1.3 | - |
pH 7.7, 46°C | Niallia circulans | D-glucose 6-phosphonate | |
2.8 | - |
pH 7.7, 46°C | Niallia circulans | D-glucose 6-homophosphonate |
General Information | Comment | Organism |
---|---|---|
evolution | 2-deoxy-scyllo-inosose synthase, i.e. DOIS or BtrC, clearly belongs to the family of dehydroquinate synthase, DHQS | Niallia circulans |
metabolism | key enzyme in the biosynthesis of 2-deoxystreptamine-containing aminocyclitol antibiotics | Niallia circulans |