Application | Comment | Organism |
---|---|---|
medicine | the decrease of this enzyme is the most frequent cause of atypical phenylketonuria | Bombyx mori |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
monoiodoacetate | - |
Bombyx mori | |
NEM | - |
Bombyx mori | |
PCMB | - |
Bombyx mori |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0128 | - |
7,8-dihydroneopterin triphosphate | pH 8.5, 37ºC, 1 mM Mg2+ | Bombyx mori | |
0.0169 | - |
7,8-dihydroneopterin triphosphate | pH 8.5 37ºC, 5 mM Mg2+ | Bombyx mori |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | stimulatory effect, 5 mM greatly accelerates activity | Bombyx mori | |
Ca2+ | stimulatory effect and additive effect at Mg2+, 1 mM | Bombyx mori | |
Mg2+ | stimulates, Km of 0.2 mM and 2 mM at pH 8.5 and 37ºC | Bombyx mori | |
Mn2+ | stimulatory effect but additional competitive or inhibitory effect | Bombyx mori | |
additional information | Cu2+ has no effect on activity | Bombyx mori | |
Ni2+ | no effect on enzyme activity | Bombyx mori | |
Sr2+ | 5 mM greatly accelerates activity | Bombyx mori | |
Zn2+ | no effect on enzyme activity | Bombyx mori |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18500 | - |
4 * 18500, SDS-PAGE | Bombyx mori |
72000 | - |
native PAGE | Bombyx mori |
85000 | 87000 | gel filtration | Bombyx mori |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bombyx mori | - |
silkworm | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, hydroxyapatite column, gel filtration | Bombyx mori |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fat body | larvae | Bombyx mori | - |
larva | fat bodies | Bombyx mori | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,8-dihydroneopterin triphosphate | - |
Bombyx mori | 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 18500, SDS-PAGE | Bombyx mori |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Bombyx mori |